西安交通大学学报（医学版）2024，Vol.45Issue(4)：567-574,8.DOI:10.7652/jdyxb202404007
急性心肌梗死发病过程中miR-30a-5p的变化及其潜在的分子机制
Changes of miR-30a-5p during the pathogenesis of acute myocardial infarction and its potential molecular mechanisms
摘要
Abstract
Objective To evaluate the potential of miR-30a-5p as a novel indicator of acute myocardial infarction(AMI)and the underlying molecular mechanisms.Methods AMI-associated microRNAs(miRNAs)and mRNA microarray datasets were downloaded from the GEO database.Real-time fluorescence quantitative PCR(qRT-PCR)technique was used to detect the level of miRNAs in serum samples;automatic biochemistry was used to detect other biochemical indicators.Receiver operating characteristic(ROC)curve analysis and Spearson correlation analysis were performed to assess the value of miR-30a-5p as a diagnostic AMI marker.The target genes of miR-30a-5p were predicted with the R language multiMiR package,and the protein interaction network was constructed by the STRING database.The results were imported into Cytoscape 3.7.1 software to screen the pivotal genes of the network.The R language clusterProfiler package performed KEGG and GO analyses on the hub genes to explore the clinical significance of miR-30a-5p in AMI and its potential molecular mechanisms.Results Compared with the control group,miR-30a-5p was upregulated significantly in the serum of patients in the AMI group(P＜0.05);miR-30a-5p was positively correlated with the levels of CK-MB,CK,TnT,proBNP and CRP(rs=0.489,P＜0.001;rs=0.347,P＜0.001;rs=0.545,P＜0.001;rs=0.533,P＜0.001;rs=0.206,P＜0.05).The area under the ROC curve was 0.862,with sensitivity of 84.4％and specificity of 74.2％.A total of 780 differentially expressed genes(DEGs)were obtained from the two datasets.A total of 1 061 target genes of miR-30a-5p and 61 common genes were identified by taking the intersection set.Among the central genes of PPI,BCL6,FOSL2,JDP2,LYN,PDE4D,SOCS3 and SOX4 scored high and were closely associated with the occurrence of AMI.KEGG and GO enrichment analyses showed that miR-30a-5p might regulate JAK-STAT,NF-kappaB and Wnt signaling pathways,which were involved in inflammatory response,apoptosis,autophagy and post-infarction remodeling,and thus participated in the process of AMI.Conclusion Serum miR-30a-5p is up-regulated in the early stage of AMI,and the study on miR-30a-5p and its regulatory pathways can help with the diagnosis and treatment of myocardial infarction.关键词
基因表达数据库(GEO)/急性心肌梗死(AMI)/microRNAs(miRNAs)/生物信息学Key words
Gene Expression Omnibus(GEO)/acute myocardial infarction(AMI)/microRNAs(miRNAs)/bioinformatics分类
医药卫生引用本文复制引用
梁国新,郭畅,唐红悦,张明明..急性心肌梗死发病过程中miR-30a-5p的变化及其潜在的分子机制[J].西安交通大学学报（医学版）,2024,45(4):567-574,8.基金项目
2021年度河北省"三三三人才工程"资助项目(No.A202105015)Supported by the 2021 Hebei Province"Three Thirty-Three Talents Project"Funding Program(No.A202105015) （No.A202105015）
