青岛市3种常绿灌木滞尘量与叶微观特征及光合作用等的相关性分析OA北大核心CSTPCD
Correlation Analysis Between Dust Retention and Leaf Microscopic Characteristics and Chlorophyll Fluorescence Parameters of Three Evergreen Shrubs in Qingdao
为探讨滞尘后3种灌木叶片叶绿素含量、叶绿素荧光参数以及气体交换参数的变化,分析叶面微结构以及叶绿素荧光参数、气体交换参数与植物滞尘量的相关性.以青岛市城阳区3种常见的常绿灌木为研究对象,通过3级滤膜过滤法测定各灌木单位叶面积滞留不同粒径颗粒物(TSP、PM>10、PM10、PM2.5)的质量,通过扫描电镜观察比较3种常绿灌木的叶表面微形态特征.结果表明,1)3种灌木叶片单位叶面积滞尘能力为火棘>瓜子黄杨>小叶女贞.2)电镜扫描叶表面微结构可知,叶表面粗糙、下表皮气孔较多且分布密集,起伏大,存在不规则条纹组织及沟状组织的植物有利于颗粒物的附着,主成分分析发现3种灌木的单位叶面积TSP仅与植物的下表皮沟槽宽度呈现出正相关关系,与其他指标无显著相关性.3)滞尘后3种灌木叶片叶绿素含量、最大光化学效率(Fv/Fm)和光化学猝灭系数(qP)显著(P<0.05)低于滞尘前处理,而非光化学猝灭系数(NPQ)则显著高于滞尘前处理;此外,3种灌木叶片净光合速率(Pn)、蒸腾速率(Tr)、气孔导度(Gs)和胞间CO2浓度(Ci)差异显著,与滞尘前相比,滞尘后3种灌木的Pn、Tr、Gs分别下降27.1%、44.2%、7.1%,7.9%、38.3%、19.7%和 38.0%、38.7%、11.6%,Ci分别增加 8.2%、5.1%、4.9%,主成分分析可知,3种灌木的单位叶面积TSP与叶绿素含量、Fv/Fm以及Pn呈显著或极显著负相关,与C,呈显著正相关.结论认为,叶表面粗糙度、气孔数量及大小、沟槽宽度等影响植物的滞尘能力,滞尘对植物叶片叶绿素以及叶绿素荧光参数产生一定程度的影响,降低植物叶片的光合作用,后期可考虑选择火棘作为滞尘能力强的灌木树种.
Taking three common evergreen shrubs in Chengyang District,Qingdao City as the research ob-jects,the mass of particulate matter(TSP,PM>10,PM10,PM2.5)retained per unit leaf area of each shrub was measured using a three-stage membrane filtration method.The leaf surface micromorphological char-acteristics of the three evergreen shrubs were observed and compared under scanning electron microscopy,and the correlations between leaf microstructure,chlorophyll fluorescence parameters,gas exchange param-eters,and plant dust retention were analyzed.The dust retention capacity per unit leaf area of the leaves of the three shrubs was as follows:Pyracantha fortuneana>Buxus sinica>Ligustrum quihoui.Scanning the microstructure of the leaf surface with an electron microscope showed that the leaf surface was rough,with a dense and undulating distribution of stomata in the lower epidermis.Plants with irregular striped and fur-rowed tissue were conducive to the attachment of particles.Principal component analysis found that the TSP per unit leaf area of the three shrubs only showed a positive correlation with the width of the grooves in the lower epidermis of the plant,and there was no significant correlation with other indicators.The chlo-rophyll content,maximum photochemical efficiency(Fv/Fm),and photochemical quenching coefficient(qP)of the leaves of the three shrubs after dust retention were significantly lower than those before dust retention,while the non photochemical quenching coefficient(NPQ)was significantly higher than those be-fore dust retention.In addition,there were significant differences in the net photosynthetic rate(Pn),tran-spiration rate(Tr),stomatal conductance(Gs),and intercellular CO2 concentration(Ci)of the leaves of the three shrubs.Compared with those before dust retention,the Pn,Tr,and Gs of the three shrubs after dust retention decreased by 27.1%,44.2%,7.1%,7.9%,38.3%,19.7%,and 38.0%,38.7%,11.6%,and Ci increased by 8.2%,5.1%,and 4.9%,respectively.The principal component analysis showed that the TSP and chlorophyll content per unit leaf area of the three shrubs Fv/Fm and Pn had a significant or ex-tremely significant negative correlation,and a significant positive correlation with Ci.The conclusion is that leaf surface roughness,number and size of stomata,and groove width affect the dust retention ability of plants,and dust retention have a certain degree of impact on chlorophyll and chlorophyll fluorescence pa-rameters of plant leaves,reducing photosynthesis of plant leaves.P.fortunei can be considered as a shrub species with strong dust retention ability.
孙丽;张颖;李文彬;包红光;孙迎坤
青岛农业大学 园林与林学院,山东青岛 266109日照园林环卫集团,山东日照 276800内蒙古农业大学林学院,内蒙古呼和浩特 010018
生物学
滞尘量叶微观特征叶绿素荧光气体交换参数
dust retention capacityleaf microscopic characteristicschlorophyll fluorescencegas exchange parameter
《西北林学院学报》 2024 (004)
232-241 / 10
山东省自然科学基金项目(ZR2023MC156).
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