西北植物学报2024，Vol.44Issue(7)：1046-1054,9.DOI:10.7606/j.issn.1000-4025.20230678
外源IBA对无花果扦插苗抗氧化特性及IAA生物合成途径的影响
Effects of exogenous IBA on antioxidant property and IAA biosynthesis pathway in cuttings of Ficus carica
摘要
Abstract
[Objective]Exploring the effects of suitable concentrations of indole butyric acid(IBA)on roo-ting and sprouting as well as on antioxidant property and gene expression related to growth hormone bio-synthesis pathway of fig(Ficus carica L.)cuttings may provide theoretical basis for its application in fig breeding,propagation,promotion,and planting.[Methods]The hard branches of'Bojihong'fig variety were used as cuttings to analyze the effects of IBA treatments(0,30,45,60,90 mg/L)on the rooting traits and antioxidant properties of the scions,as well as analyzed the transcriptome of axillary buds in the middle part of fig cuttings under 45 mg/L IBA treatment.[Results](1)The germination and rooting rates of fig spikes reached the maximum at 45 mg/L IBA and differed from other treatments and the control.(2)With the increase of IBA concentrations,the SOD and CAT activities of spikes showed a trend of first decreasing and then increasing,and both were lower than the control at 45 and 60 mg/L IBA,while POD activity was unchanged between the treatment and control.MDA and H2O2 contents in the spikes under IBA treatments were higher than the control,and MDA content in the spikes at 45 mg/L IBA was lower than other treatments.(3)Transcriptome analysis showed that there were 6 879 differentially expressed genes at 45 mg/L IBA.KEGG enrichment showed that there were 10 differential pathways.GO enrich-ment analysis indicated that the biological processes and molecular functions were the main biological pathways;the genes related to CAT and SOD were enriched in peroxisomal pathway,while the genes re-lated to POD were enriched in phenylpropane biosynthetic pathway.The metabolism-related gene FcGH3 was up-regulated in the IAA biosynthesis pathway,and the genes related to signaling,such as FcAUX1,FcARG7,and FcARF,were down-regulated.[Conclusion]IBA treatment induced the expression of genes encoding antioxidant enzymes and genes involved in IAA biosynthesis pathway of fig spikes,enhanced resistance to stress,and promoted rooting,sprouting,and seedling formation and 45 mg/L IBA had the best effect.关键词
无花果/扦插苗/吲哚丁酸(IBA)/抗氧化/IAA生物合成Key words
fig(Ficus carica L.)/cuttings/indole butyric acid(IBA)/antioxidant/IAA biosynthesis分类
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陈紫玉,巴哈依丁·吾甫尔,任桂霖,魏靖,王梓然..外源IBA对无花果扦插苗抗氧化特性及IAA生物合成途径的影响[J].西北植物学报,2024,44(7):1046-1054,9.基金项目
云南省基础研究专项-青年项目(202101AU070094) （202101AU070094）
云南省基础研究专项-面上项目(202301AT07049) （202301AT07049）
