西北植物学报2024，Vol.44Issue(7)：1094-1104,11.DOI:10.7606/j.issn.1000-4025.20230736
叶面喷水影响芒果叶片"午休"的转录组分析
Transcriptome analysis of the effects of foliar water application on the nap time of mango leaves
摘要
Abstract
[Objective]In order to reveal the molecular mechanism of photosynthetic midday-depression broken by water spring,the effects of continuous spraying on mango leaves were studied by omics technol-ogy.This study can provide basis for further optimizing water management to achieve the best conse-quences,and will have theoretical significance in enhancing mango yield and quality by aligning with the national policy objectives of reducing fertilizer usage and improving efficiency.[Methods]Mango cultivar"Tainong No.1"in Yuanjiang was used as material.The mango in the fruiting stage was sprayed by water for three times during the midday depression(12:30-14:10),20 minutes each time,with no water spra-ying as the control.The leaves were collected for transcriptome sequencing analysis.GO and KEGG data-bases were used to analyze the differentially expressed genes(DEGs).Analysis of gene expression was conducted by using DESeq,with|log2αFC|＞1 and P＜0.05 for DEG screening.[Results]3 789,2 885,and 1 667 differentially expressed genes were obtained from three development stages,respectively.GO analysis found that the DEGs were highly correlated with the membrane,intrinsic components of the membrane,components of the membrane and other items in the classification of cell components.KEGG analysis showed that the DEGs were mainly enriched in the pathways of plant hormone signal transduc-tion,MAPK signal pathway,circadian rhythm,metabolism of amino sugar and nucleotide sugar,fla-vonoid biosynthesis,photosynthetic biological carbon fixation,biosynthesis of cutin,sulfite,and wax,metabolism of porphyrin and chlorophyll,starch and sucrose metabolism,and glyceride metabolism.Ac-cording to the enrichment results,eight differentially expressed genes were screened.With qRT-PCR veri-fication,the gene expression trend was generally consistent with the results of RNA-seq.[Conclusion]The delayed expression of CAO and POR genes suggests that water spraying can prolong chlorophyll syn-thesis time.Expression of genes encoding FBP and SBP is slightly decreased,suggesting that water spray may regulate gene expression and ease photosynthetic"midday-depression",which is beneficial to carbon assimilation and photosynthetic efficiency.关键词
芒果/光合午休/转录组/叶绿素生物合成/光合生物碳固定Key words
mango/photosynthetic midday-depression/transcriptome/biosynthesis of chlorophyll/car-bon fixation in photosynthetic organisms分类
农业科技引用本文复制引用
丁雨格,侯琪,周玲,计珠琳,吕鹏悦,彭磊..叶面喷水影响芒果叶片"午休"的转录组分析[J].西北植物学报,2024,44(7):1094-1104,11.基金项目
云南省重大科技专项——绿色食品国际合作研究中心课题(2019ZG00907) （2019ZG00907）
云南省基础研究专项重大项目(202101BC070002) （202101BC070002）
