西北植物学报2024，Vol.44Issue(7)：1105-1112,8.DOI:10.7606/j.issn.1000-4025.20230631
草莓FaWRKY70基因克隆与表达模式分析
Cloning and expression analysis of the FaWRKY70 gene in strawberry
摘要
Abstract
[Objective]WRKY is one of the plant specific transcription factor families,involved in various plant life activities.However,currently there are few reports related to the WRKY70 gene in strawberry.Analyzing the role of WRKY70 homologous genes in strawberry in response to stress will help accelerate the application of molecular breeding technology and cultivate new strawberry germplasm resources.[Methods]The FaWRKY70 gene was cloned from'Benihoppe'strawberry fruits using homologous clo-ning method.Its conserved domain,physicochemical properties,protein structure,and evolutionary rela-tionship were analyzed using bioinformatics.Expression pattern analysis was performed using qRT-PCR.[Results]The FaWRKY70 gene had a length of 1 020 bp and encodes 339 amino acids.Homologous gene alignment revealed that FaWRKY70 had a high degree of amino acid sequence similarity with species in the same family such as apple and peony.The homologous genes were mostly related to plant responses to bi-otic and abiotic stress,suggesting that FaWRKY70 may be involved in resistance to stress.FaWRKY70 was expressed in different organs of strawberry,with significant differences.The expression level was highest in flowers and lowest in fruits.Under salicylic acid treatment,the FaWRKY70 gene was quickly responded,reaching its highest expression level after 3 hours,and gradually decreased thereafter.Under the treatment with methyl jasmonate,the FaWRKY70 gene showed a slight degree of induction and an overall downward trend.[Conclusion]FaWRKY70 is involved in growth and hormone signal transduction through different response modes in strawberry.关键词
草莓/FaWRKY70/基因克隆/差异表达Key words
strawberry/FaWRKY70/gene cloning/differential expression分类
生物科学引用本文复制引用
邵妍丽,卢贝,贾思振,汤伟华,廖云飞..草莓FaWRKY70基因克隆与表达模式分析[J].西北植物学报,2024,44(7):1105-1112,8.基金项目
西藏自治区自然科学基金项目(XZ202201ZR0004G) （XZ202201ZR0004G）
江苏农林职业技术学院科技项目(2023kj23) （2023kj23）
