西北植物学报2024，Vol.44Issue(7)：1122-1128,7.DOI:10.7606/j.issn.1000-4025.20230744
慈姑基因组大小鉴定及核型分析
Genome size identification and karyotype analysis of Sagittaria tri folia
摘要
Abstract
[Objective]Sagittaria trifolia is an important horticultural and cash crop in China.Identifica-tion of genome size and analysis of karyotype are essential for genome research and molecular genetics of S.tri folia.Currently,the genome size and chromosome morphology of S.tri folia are unclear.The present study provides cytological references to S.tri folia breeding and species evolution analysis.[Methods]Using flow cytometry and fluorescence in situ hybridization,together with reference to the ly-sate formulation to probe configuration,the genome size of the root tips of S.trifolia variety'Zijinxing'(ZJX)was identified and the chromosome morphology was analyzed.[Results](1)The tested cecropia was a diploid plant,and the genome of'Zijinxing'cecropia was about 16 Gb.(2)Using DAPI fluores-cence staining,the number of chromosomes was identified to be stable,consistent with the results of telo-meric fluorescence in situ hybridization.The chromosome types were middle mitotic(metacentric chromo-some),near-middle mitotic(submetacentric chromosome),and proximal mitotic(acrocentric chromo-some),with the most centromere was found in the proximal part,and no satellite was found.The karyo-type formula was 2n=2x=22=6m+4sm+12st,belonging to the type 3B of Stebbins,with the asymme-try coefficient of 99.13.[Conclusion]The study concludes that the genome of S.trifolia is of medium size,with a highly asymmetric karyotype and a high degree of evolution.关键词
慈姑/基因组大小/荧光原位杂交/核型分析Key words
Sagittaria trifolia/genome size/FISH/karyotype analysis分类
农业科技引用本文复制引用
周子琦,孙楠,严雅洁,杨志远,李良俊,冯凯..慈姑基因组大小鉴定及核型分析[J].西北植物学报,2024,44(7):1122-1128,7.基金项目
国家特色蔬菜产业技术体系项目(CARS-24) （CARS-24）
江苏省种业振兴"揭榜挂帅"项目[JBGS(2021)017] （2021）
