西北植物学报2024，Vol.44Issue(7)：1164-1168,5.DOI:10.7606/j.issn.1000-4025.20230850
利用冰冻切片机快速制作木质茎的显微切片技术
A rapid cryo-sectioning method for micro-structural observation of woody stems
摘要
Abstract
[Objective]The woody stems of plants are difficult to obtain satisfactory microscopic image in both freehand and paraffin sections due to the high content of secondary vascular tissues and high degrees of lignification of cell walls.Microtome cryostat can section the tissue with a certain hardness.It will be one of the effective tools for the section of plant woody stems.[Methods]Some woody stems with uniform texture and high water content can be sectioned by cryo-sectioning without chemical fixation.For stems with hard wood and less water content,observation was performed after fixation with FAA and other fixa-tion,OCT embedding,(-30)-(-25)℃ sectioning,and staining with 0.1％toluidine blue staining so-lution.[Results]For tree or shrub stems with a diameter of less than 6 cm,cryo-sectioning method could be used to quickly obtain complete structure and clear and colorful microstructure images.[Conclusion]The microtome cryostat can be used for microstructure sectioning of woody stems combined with toluidine blue staining,which has the advantage of simplicity,efficiency,and ease of operation.关键词
木质茎/冰冻切片/显微结构Key words
woody stems/cryo-sectioning/microstructure分类
农业科技引用本文复制引用
马芳,宋玉霞,刘文哲..利用冰冻切片机快速制作木质茎的显微切片技术[J].西北植物学报,2024,44(7):1164-1168,5.基金项目
国家自然科学基金面上项目(32371693) （32371693）
宁夏农林科学院农业高质量发展和生态保护科技创新示范项目"特色资源植物利用与沙产业技术研发" （）
