现代医药卫生2024，Vol.40Issue(14)：2349-2352,4.DOI:10.3969/j.issn.1009-5519.2024.14.003
基于高通量表面等离子体共振技术筛选与TPBG具有高亲和力的单克隆抗体研究
Screening study of monoclonal antibodies with high affinity to TPBG based on high-throughput surface plasmon resonance technology
摘要
Abstract
Objective To investigate a method of rapidly screen monoclonal antibodies with high affini-ty to trophoblastic glycoprotein(TPBG)from the prepared monoclonal antibodies.Methods High-through-put surface plasmon resonance(SPR)technology was used to immobilize TPBG of different species on the GLC sensor chip by amino coupling.Gradient dilution of the antibody was used as the flow phase to screen monoclonal antibodies that can highly affinity TPBG,and the kinetic constants of the interaction were deter-mined.Antibody 1 binds to human and cynomolgus monkey TPBG with affinity of 65.0 and 31.6 nmol/L,re-spectively.Antibody 6 binds to human,mouse and cynomolgus monkey TPBG with affinity of 77.6,92.1 and 87.7 nmol/L,respectively.Results This study successfully screened two monoclonal antibodies that can spe-cifically bind to TPBG.Meanwhile,the specific binding of these two antibodies to TPBG protein was stable by binding kinetic.Conclusion The monoclonal antibodies with high affinity to TPBG protein are screened out by SPR,which provides a method for the development of some new anti-tumor drugs.关键词
5T4蛋白/高通量/表面等离子体共振/亲和力/单克隆抗体/抗肿瘤药物Key words
TPBG protein/High-throughput/Surface plasmon resonance/Affinity/Mono-clonal antibodies/Anti-tumor drug分类
医药卫生引用本文复制引用
江秀玲,王晓丽,胡有根,彭建明,严慧深..基于高通量表面等离子体共振技术筛选与TPBG具有高亲和力的单克隆抗体研究[J].现代医药卫生,2024,40(14):2349-2352,4.基金项目
江苏省自然科学基金面上项目(18KJD36003) （18KJD36003）
扬州市职业大学校级自然科学基金项目(2018ZR28). （2018ZR28）
