新发传染病电子杂志2024，Vol.9Issue(3)：78-85,8.DOI:10.19871/j.cnki.xfcrbzz.2024.03.017
肠杆菌目细菌碳青霉烯酶检测的研究进展
Advances in the detection of carbapenemase among Enterobacterales
摘要
Abstract
In recent years,bacterial drug resistance has become a major challenge to public health,with carbapenem-resistant Enterobacteriaceae(CRE)being particularly resistant to antibiotics.The main resistance mechanism of CRE is the production of carbapenemases,which are carried differently by different strains and encode different resistance genes;therefore,early detection of carbapenemases and resistance genes plays an important role in controlling the spread of drug-resistant bacteria.The current phenotypic assays for carbapenemases are the modified Hodge assay,the carbapenem inactivation assay,the Carba NP assay,the immunological assay,and mass spectrometry.For the detection of genotypes,there are real-time fluorescence quantitative PCR assay microarray technology,isothermal amplification assay and sequencing technology,all of which are widely used in the detection of carbapenemases.Understanding the advantages and disadvantages of each detection technology is very important for the selection of the most appropriate detection method in practical applications,this paper focuses on the research progress of CRE carbapenemase detection methods and the advantages and disadvantages of each method are reviewed,to provide data support for the detection and prevention of CRE as well as the control of nosocomial infections.关键词
碳青霉烯类耐药肠杆菌目细菌/碳青霉烯酶/诊断/检测Key words
Carbapenem resistant Enterobacteriaceae/Carbapenemase/Diagnosis/Detection分类
医药卫生引用本文复制引用
滑少为,赵子今,赵萌,申辛欣,冯志山..肠杆菌目细菌碳青霉烯酶检测的研究进展[J].新发传染病电子杂志,2024,9(3):78-85,8.基金项目
国家重点研发计划(2921YFC2301100) （2921YFC2301100）
