新疆农业科学2024，Vol.61Issue(4)：984-995,12.DOI:10.6048/j.issn.1001-4330.2024.04.023
双斑长跗萤叶甲雌虫、雄虫触角转录组及差异表达基因分析
Analysis of the antennal transcriptome and differentially expressed genes of female and male Monolepta signata
摘要
Abstract
[Objective]To establish the transcriptome database of adult Monolepta signata antennae,screen the differentially expressed genes in the antennae of female and male M.signata and analyze the physi-ological functions of these genes.[Methods]In this study,Illumina high-throughput sequencing technology was used to conduct transcriptome sequencing on the antennae of male and female M.signata and analyze the functional annotation and pathway enrichment of unigenes,and after that the expression level of odorant bind-ing protein genes in antennae was detected by RT-qPCR.[Results]A total of 73,050 unigenes were ob-tained from the antennae transcriptome sequencing of female and male of adult M.signata,with the percentage of Q30 bases above 93.23,and 34,233 unigenes were annotated in at least one database(46.86%).A total of 395 differentially expressed genes were detected in the antennae of male M.signata compared with that of female M.signata.Among them,288 genes were up-regulated and 107 genes were down-regulated.[Con-clusion]In the GO database,the most significant function of enrichment of these differentially expressed genes were odorant binding,olfactory receptor activity,and sensory perception of smell.RT-qPCR results showed that the relative expression levels of odorant binding protein genes in antennae of female and male M.signata were consistent with the transcriptome sequencing results.The transcriptome database of the antenna of adult M.signata is successfully obtained.关键词
双斑长跗萤叶甲/触角转录组/功能注释/差异表达基因Key words
Monolepta signata/antennae transcriptome/functional annotation/differentially expressed genes分类
农业科技引用本文复制引用
何婉洁,孟涵颖,支梦婷,陈静..双斑长跗萤叶甲雌虫、雄虫触角转录组及差异表达基因分析[J].新疆农业科学,2024,61(4):984-995,12.基金项目
新疆维吾尔自治区重点研发任务专项(2022B02043-2) （2022B02043-2）
国家自然科学基金项目(31960541,31460473) （31960541,31460473）
新疆生产建设兵团指导性科技计划项目(2022ZD010) Supported by the Key R&D Task Special Project of Xinjiang Uygur Autonomous Region(2022B02043-2) （2022ZD010）
National Natural Sci-ence Foundation of China(31960541,31460473) （31960541,31460473）
The Guiding Science and Technology Program Project of Xinjiang Production an Construction Corps(2022ZD010) （2022ZD010）
