头孢菌素菌渣有机肥对玉米土壤中耐药菌及相关抗性基因的影响
Effect of cephalosporin residue organic fertilizer on resistant bacteria and related resistance genes in maize soil
摘要
Abstract
[Objective]To explore the effect of cephalosporin residue organic fertilizer on the abundance of drug-resistant bacteria and related resistance genes in the maize soil,and to provide scientific basis for bi-ological safety evaluation in the application of cephalosporin residue organic fertilizer.[Methods]Three ex-perimental groups were set up,which included none organic fertilize treatment(CK),the organic fertilizer treatments with 500 kg/667 m2(B1)and with 1000 kg/667 m2(B2),respectively.The antibiotic resistant bacteria and related antibiotic resistance genes(ARGs)with different treatments were analyzed by using the cultivable method and the real-time fluorescence quantitative PCR.[Results]The results showed that the to-tal number of bacteria was significantly increased in the treatments with cephalosporin residue organic fertiliz-er,and the number of cefradine resistant bacteria was significantly increased in the treatments at corn seedling stage as well.Forty-one strains of antibiotic-resistant strains were obtained,among which 8 cefradine resist-ant strains belonged to 6 genera.[Conclusion]The relative abundance of ARGs genes in corn seedling soil was significantly increased in the treatments with cephalosporin residue organic fertilizer.There were no signifi-cant effects on the relative abundance of bla TEM gene in the treatments at the fruiting stage,and none regular influence on other drug-resistant genes.关键词
头孢菌素菌渣/玉米土壤/耐药菌/ARGs/q-PCRKey words
Cephalosporin residue/corn soil/drug-resistant bacteria/ARGs/q-PCR分类
农业科技引用本文复制引用
刘婉琴,易鸳鸯,彭小武,谢芳,顾美英,张志东..头孢菌素菌渣有机肥对玉米土壤中耐药菌及相关抗性基因的影响[J].新疆农业科学,2024,61(4):1003-1010,8.基金项目
新疆维吾尔自治区青年科技拔尖人才项目-青年科技创新人才培养(2022TSYCCX0067) （2022TSYCCX0067）
新疆农业科学院科技创新重点培育专项(xjkcpy-2021002) （xjkcpy-2021002）
新疆农业科学院自主培育项目团队建设专项(nkyzztd-001) （nkyzztd-001）
新疆农业科学院农业科技创新平台能力提升建设专项(nkypt005) Xinjiang Uygur Autonomous Region Young Science and Technology Top Talent project-young Science and technology innovation personnel training(2022TSYCCX0067) （nkypt005）
Key Science and Technology Innovation Incubation Project of Xinjiang Academy of Agricul-tural Sciences(xjkcpy-2021002) （xjkcpy-2021002）
Independent Cultivation Project Team Construction Special Project of Xinjiang Academy of Agri-cultural Sciences(nkyzztd-001) （nkyzztd-001）
Agricultural Science and Technology Innovation Platform Capacity Improvement Project of Xin-jiang Academy of Agricultural Sciences(nkypt005) （nkypt005）
