新疆农业科学2024，Vol.61Issue(4)：1011-1020,10.DOI:10.6048/j.issn.1001-4330.2024.04.026
一株群体感应抑制活性链霉菌的筛选鉴定及其毒力因子代谢能力分析
Screening and identification of a Streptomyces strain with quorum sensing inhibitory activity and effect of the crude extracts on virulence factors of Erwinia amylovora
摘要
Abstract
[Objective]To explore the potential metabolisms for natural product compounds from actino-mycetes in the arid area and investigate effect of the quorum sensing inhibition on virulence factors in Erwinia amylovora.[Methods]Chromobacterium violaceum 026(CV026)was used for the screening model of the quorum sensing inhibitions,the fermentation of actinomycetes isolated from soils in arid areas around the Ku-mutag Desert was carried out,and the quorum sensing inhibitory activity of the fermentation broth was tested u-sing the Oxford Cup method.The strain was identified by the 16S rRNA gene sequence analysis,and effect of crude extracts from the broth on virulence factors of Erwinia amylovora was measured.In addition to that,liq-uid chromatography and mass spectrometry(LC-MS)was used to measure metabolites,and compounds with similar structure to reported quorum sensing inhibitors were selected to validate.[Results]Isolate K-9 was screened and identified to belong to the genus Streptomyces,which showed strong quorum sensing inhibitory ac-tivity and shared the highest similarities with Streptomyces rubradiris NBRC 14000T(99.72%).The crude ex-tracts of the broth effectively reduced the violacein production of CV026 and the swimming ability of Erwinia amylovora.Virulence factors of Erwinia amylovora such as biofilm,pyocyanin and rhamnolipid were inhabited significantly with the concentration-dependent interactions.A total of 635 compounds were identified from the crude extract by LC-MS,and 5 compounds with significant quorum sensing inhibitory activity were observed.[Conclusion]Streptomyces sp.K-9 is one potential new species isolated from arid areas,whose fermentation metabolites shows obvious quorum sensing inhibitory activity,and can inhibit virulence factors produced by Pseudomonas aeruginosa.Additionally,many kinds of novel quorum sensing inhibitors were obtained.关键词
群体感应抑制剂/干旱地区放线菌/欧文氏菌/毒力因子Key words
quorum sensing inhibition/actinomycete from arid area/Erwinia amylovora/virulence fac-tors分类
农业科技引用本文复制引用
柏晓玉,朱丽英,包慧芳,江凌,朱静,顾美英,朱艳蕾,张志东..一株群体感应抑制活性链霉菌的筛选鉴定及其毒力因子代谢能力分析[J].新疆农业科学,2024,61(4):1011-1020,10.基金项目
新疆维吾尔自治区青年科技拔尖人才项目-青年科技创新人才培养(2022TSYCCX0067) （2022TSYCCX0067）
新疆农业科学院科技创新重点培育专项(xjkcpy-2021002) （xjkcpy-2021002）
国家自然科学基金项目(32060004) （32060004）
新疆维吾尔自治区自然科学基金项目"杰出青年基金"(2022D01E19) Xinjiang Uygur Autonomous Region Young Science and Technology Top Talent project-young Science and technology innovation personnel training(2022TSYCCX0067) （2022D01E19）
Key Cultivation Project of Science and Technology Innovation by Xinjiang Academy of Agri-cultural Sciences(xjkcpy-2021002) （xjkcpy-2021002）
National Natural Science Foundation of China(32060004) （32060004）
Xinjiang Natural Science Foun-dation for Distinguished Young Scholars(2022D01E19) （2022D01E19）
