新疆农业科学2024，Vol.61Issue(4)：1021-1028,8.DOI:10.6048/j.issn.1001-4330.2024.04.027
原驼乳中乳酸菌的分离筛选及特性分析
Screening,identification and characteristics of Lactic acid bacteria from raw camel milk
摘要
Abstract
[Objective]To explore lactic acid bacteria(LAB)resources in raw camel milk.[Methods]Raw camel milk samples were collected around Nanshan mountain in Urumqi,Xinjiang and the strains were i-solated by the plate dilution method.The 16S rRNA gene sequences and morphological observation were car-ried out to clarify the taxonomic status of the strains,and edible safety characteristics such as detecting hemol-ysis and harmful metabolites.[Results]Results showed that a total of 29 LAB strains were isolated and identi-fied,which belonged to 4 species in Leuconostoc and Lentilactobacillus;the tested strains could tolerate 0.5%bile salt,5%NaCl,and showed good high temperature resistance,milk peptonization,anti-oxidation,dif-ferent enzyme activities,and antibacterial properties.None of strains were positive for hemolysis and produce nitroreductase.No biogenic amines were produced from amino acids by strains except T12.[Conclusion]The LAB obtained from camel milk in this study has good fermentation characteristics and food safety.关键词
原驼乳/乳酸菌/分离筛选Key words
raw camel milk/Lactic acid bacteria/screening分类
农业科技引用本文复制引用
孙建,李雪,楚敏,顾美英,艾尼江·尔斯满,朱静,何齐,谭慧林,张志东..原驼乳中乳酸菌的分离筛选及特性分析[J].新疆农业科学,2024,61(4):1021-1028,8.基金项目
新疆维吾尔自治区公益性科研院所基本科研业务经费项目(KY2022034) （KY2022034）
新疆维吾尔自治区自然科学基金项目(2021D01F62,2021D03002) （2021D01F62,2021D03002）
新疆区域协同创新专项(上海合作组织科技伙伴计划)项目(2020E01007) （上海合作组织科技伙伴计划）
新疆农业科学院自主培育项目团队建设专项(nkyzztd-001) Basic Scientific R&D Program of Public Welfare Research Institutions of Xinjiang Uygur Autonomous Region(KY2022034) （nkyzztd-001）
Natural Science Foundation of Xinjiang Uygur Autonomous Region(2021D01F62,2021D03002) （2021D01F62,2021D03002）
Xinjiang Regional Collaborative Innovation Special Project(SCO Science and Technology Partnership Program)(2020E01007) （SCO Science and Technology Partnership Program）
Key Science and Technology Innovation Incu-bation Project of Xinjiang Academy of Agricultural Sciences(nkyzztd-001) （nkyzztd-001）
