新疆农业科学2024，Vol.61Issue(5)：1077-1084,8.DOI:10.6048/j.issn.1001-4330.2024.05.005
基于RNA-seq的大麦苗期抗旱相关基因的挖掘与分析
RNA-seq-based mining and analysis of drought-related genes in barley seedlings
摘要
Abstract
[Objective]To excavate the genes related to drought resistance in the hope of providing theo-retical support for analyzing the molecular drought resistance mechanism of barley and guiding the drought re-sistance breeding of barley.[Methods]Taking New beer No.6 as the test material,we applied transcriptome sequencing RNA-seq technology to sequence the inverted bilobed leaves of barley seedlings before and after drought stress,and real-time fluorescence quantitative RT-PCR was used to verify the functional genes.[Results](1)In this study,3835 differentially expressed genes were detected in the inverted bilobed of New beer 6 before and after drought stress by using RNA-seq,mainly genes encoded by ABC transporter proteins,ribosomal proteins,transcription factors,dehydrins,peroxidases,protein phosphatases,etc.The results showed that DEG was mainly enriched in starch and sucrose metabolism,transporter proteins,plant hormone signaling transduction,and protein phosphatases.(2)By KEGG metabolic pathway enrichment analysis,DEGs were mainly enriched in starch and sucrose metabolism,transporter proteins,phytohormone signaling,chaperones and folding catalysts,aminosugar and ribosugar metabolism,phenylalanine metabolism,taurine and hyposulphite metabolism,peroxisomes and other pathways.[Conclusion]Significant differences are shown in gene expression before and after drought stress in barley.The results of this study have laid the foun-dation for the excavation of key drought-resistant genes and for further analysis of drought-resistant mecha-nisms in barley(1592 up-regulated genes and 2243 down-regulated genes).关键词
大麦/干旱胁迫/转录组/基因挖掘Key words
barley/drought stress/transcriptome/gene mining分类
农业科技引用本文复制引用
鞠乐,齐军仓,牛银亭,石培春,宋瑞娇,宋凌宇,阴志刚,陈培育,强学兰..基于RNA-seq的大麦苗期抗旱相关基因的挖掘与分析[J].新疆农业科学,2024,61(5):1077-1084,8.基金项目
财政部和农业农村部:国家现代农业产业技术体系项目(CARS-05-19B) （CARS-05-19B）
国家自然科学基金项目(32360456) The Earmarked Fund for Modern Agro-industry Technology Research System(CARS-05-19B) （32360456）
National Natural Science Foun-dation of China(32360456) （32360456）
