畜牧兽医学报2024，Vol.55Issue(7)：3075-3084,10.DOI:10.11843/j.issn.0366-6964.2024.07.026
三株新型鸭源微RNA病毒分离毒株的全基因组序列分析
Isolation and Identification of Novel Picornavirus from Ducks and Whole Genome Sequence Analysis
摘要
Abstract
The aim of this study was to isolate novel duck-derived picornavirus for whole genome sequencing and genetic in order to perform whole genome sequencing and genetic evolutionary study.In 2021,we identified the presence of an unknown class of novel picornavirus infection in the diseased materials of breeding ducks and meat ducks submitted for inspection.By inoculating the diseased material into specific pathogen-free(SPF)chicken embryos,we isolated the virus.And the isolated viruses were further detected by PCR with primers designed from sequences available and sequenced the whole genome by overlapping PCR method.The amino acid sequences of each protein of the isolated virus were compared.At the same time,the sequences of the representative strains of small RNA viruses in the GenBank database were selected to draw the phylogenetic tree,and the sequences of the main proteins P1,2C and 3D were analysed in comparison with each other.Three small RNA virus strains were isolated and named 21101,21016 and 21075(GenBank ID:OQ927377-OQ927379),with genome lengths of 7 445,7 445,and 7 447 bp.These sequence encompasses an open reading frame(ORF)that encodes a total of 2 141 amino acids.The ORF can be divided into three distinct parts:P1,P2 and P3.These protein divisions align with the characteristic features observed in picornaviruses.The genetic evolutionary analysis,conducted through the utilization of whole genome sequences,demonstrated that the three isolates were positioned within the same branch as the Duck/FC22/China/2017 strain(GenBank ID:MN102111)previously separate inour laboratory and Duck/AH15/CHN/2015 strain(GenBank ID:MT681985)at the Shanghai Veterinary Research Institute.Furthermore,these isolates exhibited the highest degree of genetic similarity to the Duck hepatitis A virus(DHAV).Three strains of duck-derived picornavirus were isolated,and whole genome sequencing and genetic evolution analysis revealed that the three isolated viruses belong to the same class of the two currently known novel duck-derived picornavirus.关键词
新型微RNA病毒/分离鉴定/基因组测序/遗传进化分析Key words
novel picornavirus/isolation and identification/genome sequence/genetic evolutionary analysis分类
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李继桐,朱彤,吕俊峰,高月花,胡峰,于可响,宋敏训,王建琳,李玉峰..三株新型鸭源微RNA病毒分离毒株的全基因组序列分析[J].畜牧兽医学报,2024,55(7):3075-3084,10.基金项目
中韩国际合作项目(122014-2) （122014-2）
山东省家禽产业技术体系(SDAIT-11-01) （SDAIT-11-01）
国家自然科学基金(32002286) （32002286）
山东省自然科学基金(ZR2020QC195) （ZR2020QC195）
