畜牧兽医学报2024，Vol.55Issue(7)：3085-3093,9.DOI:10.11843/j.issn.0366-6964.2024.07.027
犬新孢子虫miRNAs的鉴定与分析
Identification and Analysis of miRNAs of Neospora caninum
摘要
Abstract
To identify specific expressed miRNAs of Neospora caninum and to investigate their expression in host cells,the samples of caprine endometrial epithelial cells(EECs)infected with tachyzoites of N.caninum at 24 and 48 h were collected.Specific expressed miRNAs of N.caninum and their in vitro expression were analyzed by using Illumina highthroughput sequencing technology and bioinformatics analysis.The effect of an up-regulated miRNA,novel3_mature,on the propagation of N.caninum in caprine EECs was also studied.The results showed that a total of 3 404 specific expressed miRNAs(155 novel predicted miRNAs)of N.caninum were identified.Of them,77(47 up-and 30 down-regulated)miRNAs were significantly differentially expressed.The consistent results were found with highthroughput sequencing by using quantitative real-time PCR analysis of seven significantly differentially expressed miRNAs randomly selected.Predictive analysis found 3 978 target genes of 61 miRNAs.Functional enrichment analysis showed that these target genes were possible involved in MAPK signaling pathway,axon guidance,focal adhesion,cAMP signaling pathway,calcium signaling pathway.Transfection of novel3_mature mimics significantly promoted the propagation of N.caninum in caprine EECs.Many specific expressed miRNAs were encoded by N.caninum and they played important roles in the propagation of N.caninum,providing a novel way to understand the intracellular survival of N.caninum.关键词
犬新孢子虫/山羊子宫内膜上皮细胞/miRNAs/novel3_mature/增殖Key words
Neospora caninum/caprine endometrial epithelial cells/miRNAs/novel3_mature/propagation分类
农业科技引用本文复制引用
李京宇,陈金铭,张明一,赵姗姗,陶德良,宋军科,杨新,樊莹莹,赵光辉..犬新孢子虫miRNAs的鉴定与分析[J].畜牧兽医学报,2024,55(7):3085-3093,9.基金项目
国家自然科学基金(32373040) （32373040）
