畜牧兽医学报2024，Vol.55Issue(7)：3193-3204,12.DOI:10.11843/j.issn.0366-6964.2024.07.037
脂肪间充质干细胞对巴马小型猪自体皮肤移植愈合过程的影响
The Effect of Adipose Mesenchymal Stem Cells on the Healing Process of Autologous Skin Transplantation in Bama Miniature Pigs
摘要
Abstract
This study aims to use adipose derived mesenchymal stem cells(ADSCs)to intervene in an experimental animal model of autologous skin transplantation in small pigs,and observe their effects on the healing process of autologous skin transplantation.The animal model of autologous skin transplantation of 9 miniature pigs was established,and the miniature pigs were randomly divided into 3 groups:model group(Model),phosphate buffer group(PBS),and adipose mesenchymal stem cell group(ADSCs).Blood and skin tissue samples were collected before surgery and 7,14,21,and 28 days after surgery,respectively,and clinical changes of grafts and surrounding tissues were observed,photographed,and recorded.The effect of ADSCs on wound healing of mini pig autologous skin transplantation was comprehensively evaluated through skin graft apparent examination,histopathological examination,blood routine examination and serum biochemical examination,skin graft tissue oxidative stress,inflammation,regeneration and blood flow recovery.The researchers observed that the skin recovery speed of ADSCs group was better than that of the other two groups,and the ADSCs group basically returned to normal skin status 28 days after surgery.In terms of oxidative stress,SOD and GSH contents in ADSCs group were significantly increased(0.01＜P＜0.05),while MDA contents were significantly decreased(0.01＜P＜0.05).In terms of inflammation,the contents of TNF-α and IL-1β in ADSCs group were significantly decreased,and IL-10 was significantly increased on 7 and 14 days after surgery(P＜0.01).In terms of skin tissue regeneration,the expression levels of VEGF,bFGF,TGFβ3 and CD31 in ADSCs group were significantly increased(0.01＜P＜0.05).In terms of blood flow recovery,the percentage of blood perfusion of ADSCs during healing was significantly higher than that of the other two groups(0.01＜P＜0.05).To sum up,ADSCs can accelerate the recovery of skin tissue pathological damage,reduce oxidative stress reaction promote skin tissue regeneration and Angiogenesis in the miniature pig autologous skin transplantation animal model.The results indicated that ADSCs had the functions of anti-oxidation,anti-inflammation and promoting tissue regeneration,and had good therapeutic effect on autologous skin transplantation of miniature pigs.This experiment provided experimental support and scientific basis for the clinical application of stem cells in autologous skin transplantation and allogeneic skin transplantation.关键词
小型猪/脂肪间充质干细胞/自体皮肤移植/创伤愈合Key words
small pigs/adipose mesenchymal stem cells/autologous skin transplantation/wound healing分类
畜牧业引用本文复制引用
吕英光,张建涛,王洪斌,焦广明,桑金芳,寇志鹏,刘涛,王月,陆翔宇,朴晨曦,马亚军..脂肪间充质干细胞对巴马小型猪自体皮肤移植愈合过程的影响[J].畜牧兽医学报,2024,55(7):3193-3204,12.基金项目
国家自然科学基金面上项目(31972757) （31972757）
