黄连防治鸭病毒性肠炎机制的网络药理学分析及动物试验验证OA北大核心CSTPCD

To explore the mechanism of Coptidis Rhizoma treating duck viral enteritis(DVE),the active ingredients and targets of Coptidis Rhizoma were firstly screened by the Traditional Chinese Medicine System Pharmacology Analysis Platform(TCMSP),and the DVE related targets and their intersection targets with Coptidis Rhizoma were analyzed and drew using the GeneCards database and Venny and Cytoscape 3.7.2 software,which constructed a"drug component disease target"network.And the protein interaction network(PPI)were established by STRING databases and their GO function and KEGG pathway were analyzed with the DAVID database.Then,the results of network pharmacology were verified by animal experiments which narmol group,virus infection group and drug intervention group,and their tissue samples were collected for histopathological observation and related cytokine determination.The results showed that 14 active components and 212 action targets of Coptidis Rhizoma were selected,including 25 targets that intersect with DVE,involving biological processes such as RNA polymerase Ⅱ promoter transcription,sequence specific DNA binding transcription factor activity,immune response,inflammatory response,and cell apoptosis,enriched in signaling pathways such as Toll like receptor signaling,inflammatory bowel disease,cytokine-cytokine receptor interaction,and herpes simplex virus infection,the core targets are IL-6,IL-10,and STAT1.By animal experiments,there were the varting degrees of pathological damage in liver,spleen and duodenum in virus infection group ducks,and IL-6 content was significantly increased compared to the normal control group ducks(P≤0.05),while the IL-10 and STAT1 contents were significantly reduced(P≤0.05).However,the pathological damage was significantly reduced in drug intervention group ducks,the levels of IL-6,IL-10,and SYAT1 tended to normal levels.These results indicated that Coptidis can effectively alleviate the pathological damage of duck tissues caused by duck viral enteritis,and its mechanism may involves various inflammatory response signaling pathways.