五味子多糖纳米硒的体外抗氧化和肠道菌群调节作用研究OA北大核心CSTPCD

The aim of this study was to explore the in vitro antioxidant property and intestinal microbiota modulation effect of Schisandra Chinensis polysaccharide-selenium nanoparticles(SCP-SeNPs).The in vitro antioxidant property of SCP-SeNPs was evaluated using ABTS+,DPPH radicals,OH-and O2-scavenging assays,and reducing power assays.Furthermore,the impact of SCP-SeNPs on the intestinal microbiota of mice was investigated using 16S rRNA high-throughput sequencing technology.The results showed that,SCP-SeNPs exhibited significant in vitro antioxidant property,with better scavenging effects on OH-and O2-radicals compared to SCP.The 16S rRNA analysis indicated that the α-diversity index of cecal microbiota in the SCP-SeNPs group was higher than that of the Blank control(BC)group(P＞0.05).The β-diversity index suggested notable differences in microbial composition between the SCP-SeNPs and BC groups.Intestinal microbiota structure analysis revealed that Bacteroidota and Firmicutes were the major constituents of the mouse intestinal microbiota.Differential analysis of microbial composition indicated that at the genus level,beneficial bacteria such as Prevotellaceae,Muribaculum,Dubosiella,and Faecalibaculum and Clostridia_UCG_014 were significantly enriched in the SCP-SeNPs group(P＜0.05).PICRUSt 2 functional prediction analysis revealed that the pathways of Proteasome,Peroxisome,Phenylalanine metabolism,and biosynthesis of Tropane,piperidine,and pyridine alkaloids were significantly enriched in the SCP-SeNPs group(P＜0.05).In conclusion,SCP-SeNPs has certain antioxidant capacity and may have the potential to regulate intestinal microbiota composition and impact microbial metabolism through the enhancement of beneficial bacteria.These results suggest that SCP-SeNPs can serve as a novel functional trace element supplement.