畜牧兽医学报2024，Vol.55Issue(7)：3255-3260,6.DOI:10.11843/j.issn.0366-6964.2024.07.043
副结核分枝杆菌重组酶介导扩增-侧向流试纸条检测方法的建立与初步应用
Establishment and Preliminary Application of Recombinase-mediated Amplification-sidestream Detection for Detection of Mycobacterium paratuberculosis
摘要
Abstract
To establish a rapid and accurate method for detection of Mycobacterium avium subsp.paratuberculosis(MAP),primers and probes were designed according to the specific multicopy insert element IS900 of MAP.A recombinase-aid amplification combined with lateral flow dipstick(RAA-LFD)method was established for visual detection of MAP in the field within 30 min at 35 ℃ after being optimized.The assay was specific with the target sequence of MAP with a detection limit up to 10 fg·μL-1.One hundred and forty-nine stool samples were tested by this method,showing 100％sensitivity with the PCR.In short,the RAA-LFD assay developed in this study was a rapid,reliable method which can be broadly used.关键词
副结核分枝杆菌/重组酶介导扩增/侧向流试纸条Key words
Mycobacterium avium subsp.paratuberculosis/recombinase-aid amplification/lateral flow dipstick分类
农业科技引用本文复制引用
南岳,龙美贞,王元智,刘一朵,周向梅..副结核分枝杆菌重组酶介导扩增-侧向流试纸条检测方法的建立与初步应用[J].畜牧兽医学报,2024,55(7):3255-3260,6.基金项目
"十四五"重点研发计划(2022YFD1800703) （2022YFD1800703）
