畜牧与兽医2024，Vol.56Issue(8)：7-14,8.
东佛里生奶绵羊精液冷冻前后代谢组学比较分析
Comparative analysis of metabonomics of semen before and after freezing in East Friensian dairy sheep
摘要
Abstract
By comparing the differences in metabonomics of East Friensian dairy sheep semen before and after freezing,the potential mech-anism of sperm freezing injury was further explored,which provided a theoretical basis for optimizing the formula of frozen diluent of dairy sheep semen.Semen collected by artificial vagina was divided into 12 groups,6 of which were semen pre-freezing groups and 6 were semen post-freezing groups.The differential metabolites were detected and analyzed by liquid chromatography-mass spectrometry(LC-MS)coupled with non-targeted metabonomics,and the metabolic pathway enrichment analysis was carried out based on the KEGG database.The results showed that a total of 4 113 metabolites were detected in the semen of the East Friensian dairy sheep.Compared with the semen pre-freezing groups,70 metabolites changed significantly in the semen post-freezing groups,the metabolites being mainly lipids and lipid-like molecules,such as glycerophospholipids,sphingolipids,and prenol lipids.In addition,according to the enrichment degree of differential me-tabolites in the metabolic pathways,two metabolic pathways were selected,namely purine metabolism and choline metabolism in cancer.In conclusion,the freezing process significantly affected the sperm membrane structure,the oxidative decomposition of lipids and the adenosine metabolism process,which led to the sperm damage and the decline of semen quality after freezing.关键词
东佛里生奶绵羊/精液冷冻/非靶向代谢组学/液相色谱质谱联用/差异代谢物/代谢通路Key words
East Friensian dairy sheep/semen freezing/untargeted metabolomics/LC-MS/differential metabolites/metabolic pathways分类
农业科技引用本文复制引用
姜璐瑶,张磊,张迎锐,贾思潼,李桂丽,崔久增,马应天,王晓飞,张希云,宋宇轩..东佛里生奶绵羊精液冷冻前后代谢组学比较分析[J].畜牧与兽医,2024,56(8):7-14,8.基金项目
金昌奶绵羊冻精制作关键技术研究与示范项目(TGZX2021-17) （TGZX2021-17）
陕西省畜禽育种"两链"融合重点专项(2022GD-TSLD-46-0202) （2022GD-TSLD-46-0202）
陕西省重点研发计划项目(2023-YBNY-130) （2023-YBNY-130）
