东佛里生奶绵羊精液冷冻前后代谢组学比较分析OA北大核心CSTPCD

By comparing the differences in metabonomics of East Friensian dairy sheep semen before and after freezing,the potential mech-anism of sperm freezing injury was further explored,which provided a theoretical basis for optimizing the formula of frozen diluent of dairy sheep semen.Semen collected by artificial vagina was divided into 12 groups,6 of which were semen pre-freezing groups and 6 were semen post-freezing groups.The differential metabolites were detected and analyzed by liquid chromatography-mass spectrometry(LC-MS)coupled with non-targeted metabonomics,and the metabolic pathway enrichment analysis was carried out based on the KEGG database.The results showed that a total of 4 113 metabolites were detected in the semen of the East Friensian dairy sheep.Compared with the semen pre-freezing groups,70 metabolites changed significantly in the semen post-freezing groups,the metabolites being mainly lipids and lipid-like molecules,such as glycerophospholipids,sphingolipids,and prenol lipids.In addition,according to the enrichment degree of differential me-tabolites in the metabolic pathways,two metabolic pathways were selected,namely purine metabolism and choline metabolism in cancer.In conclusion,the freezing process significantly affected the sperm membrane structure,the oxidative decomposition of lipids and the adenosine metabolism process,which led to the sperm damage and the decline of semen quality after freezing.