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基于生长性状和SSR遗传多样性分析的红松第二代优树选择研究OA北大核心CSTPCD

Selection of Second-generation Superior Trees of Pinus koraiensis Based on Growth Traits and SSR Genetic Diversity Analysis

中文摘要英文摘要

以18~24年生的红松初级种子园所产苗木营建的5个地块(A1、A2、K、H1、H2)人工林为研究材料,以生长指标调查和分子标记技术分析为选择方法,筛选红松二代优树.结果表明:以单株材积优势比≥1.80作为选择标准,共选出红松优良单株94株,平均入选率为0.789%.其中,A1地块平均树高6.97 m,平均胸径17.81 cm,平均单株材积0.092 m3,入选优良单株5株,入选率0.667%;A2地块平均树高9.18 m,平均胸径18.24 cm,平均单株材积0.119 m3,入选优良单株27株,入选率2.368%;K地块平均树高10.75 m,平均胸径18.65 cm,平均单株材积0.135 m3,入选优良单株3株,入选率0.213%;H1地块平均树高5.76 m,平均胸径12.77 cm,平均单株材积0.04 m3,入选优良单株26株,入选率0.303%;H2地块平均树高5.9 m,平均胸径11.15 cm,平均单株材积0.031 m3,入选优良单株32株,入选率0.382%.群体在13个SSR位点上共检测到等位基因294个等位基因数量差异较大,且群体内存在一定程度的近交几率.在0.947相似系数下,可将94个单株划分为74个同源组.为有效控制近交,进行了同源组内优良单株的二次选择,最终入选优良单株74株,并将之用作红松二代种子园的建园材料.

In this study,5 plots(A1,A2,K,H1,H2)of 18-24 years old red pine primary seed orchard were used as research materials,and growth index investigation and molecular marker analysis were used as selection methods to screen the second generation of red pine trees.The results of growth traits analysis showed that 94 ex-cellent individual plants of red pine were selected with an average selection rate of 0.789%based on the odds ra-tio of individual tree volume ≥1.80.In the A1 plot,the average tree height was 6.97 m,the average DBH was 17.81 cm,and the average tree volume was 0.092 m3.Five excellent individual plants were selected,and the selec-tion rate was 0.667%.In the A2 plot,the average tree height was 9.18 m,the average DBH was 18.24 cm,the av-erage tree volume was 0.119 m3,and 27 excellent trees were selected,the selection rate was 2.368%.In plot K,the average tree height was 10.75 m,the average DBH was 18.65 cm,and the average tree volume was 0.135 m3.Three excellent trees were selected,and the selection rate was 0.213%.In H1 plot,the average tree height was 5.76 m,the average DBH was 12.77 cm,and the average tree volume was 0.04 m3.Twenty-six excellent trees were se-lected,and the selection rate was 0.303%.H2 plot average tree height 5.9 m,average DBH 11.15 cm,average tree volume 0.031 m3,32 excellent individual plants,the selection rate of 0.382%.The results of SSR analysis showed that 294 alleles were detected in 13 SSR loci,indicating a high level of genetic diversity.Under the similarity coefficient of 0.947,94 individual plants could be divided into 74 homologous groups.In order to effectively con-trol inbreeding,74 excellent single plants were selected from homologous groups and used as garden materials for the second generation of red pine seed orchard.

冯健;张金博;杨圆圆;杜超群;徐柏松;曹颖;姚飞

辽宁省林业科学研究院,辽宁沈阳 110032本溪满族自治县清河城实验林场,辽宁本溪 117116湖北省林业科学研究院,湖北武汉 430075辽宁省林业发展服务中心,辽宁沈阳 110036

林学

红松优树SSR标记树高胸径材积

Pinus koraiensisexcellent treeSSRheight of treediameter at breast heightvolume per tree

《西南林业大学学报》 2024 (007)

1-7 / 7

国家重点研发计划(2022YFD2201800-02)资助;辽宁省农业科学院学科建设项目(2022DD217036)资助.

10.11929/j.swfu.202309016

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