信阳师范学院学报（自然科学版）2024，Vol.37Issue(3)：343-348,6.DOI:10.3969/j.issn.1003-0972.2024.03.010
大豆蛋白磷酸酶基因GmPP2C28的克隆与表达分析
Cloning and Expression Analysis of the Protein Phosphatase Gene GmPP2C28 of Soybean
摘要
Abstract
To elucidate the role of plant protein phosphatase 2C(PP2C)in the symbiotic interaction between leguminous plants and rhizobia,a protein phosphatase gene GmP P 2C28 from soybean was cloned and its encoded protein was subcellular localized.Subsequently,the recombinant protein was expressed and purified in Escherichia coli.In addition,the expression patterns of GmPP 2C28 gene in different tissues and roots at different stages after inoculation with rhizobia were analyzed using fluorescence quantitative PCR technology.The results showed that the cDNA sequence length of the complete coding region of GmPP2C28 was 1029 bp,encoding 343 amino acids,and its coding protein was located in the nucleus of Arabidopsis protoplasts.The recombinant protein in vitro was highly expressed in soluble form.The target band size of the purified GmPP2C28 protein was about 37 kD.The expression level of GmPP2C28 gene was relatively high in the root and nodule of soybean,and the expression level showed a pattern of first increasing and then decreasing in the root of soybean after inoculation with rhizobia.关键词
大豆/蛋白磷酸酶2C/共生结瘤/蛋白表达/基因表达Key words
soybean/protein phosphatase 2C/symbiosis nodulation/protein expression/gene expression分类
农业科技引用本文复制引用
柯丹霞,侯仕博,马斯羽,王坤,李佩瑶,窦梦歌,张滋益..大豆蛋白磷酸酶基因GmPP2C28的克隆与表达分析[J].信阳师范学院学报（自然科学版）,2024,37(3):343-348,6.基金项目
国家自然科学基金项目(U1904102) （U1904102）
河南省高等学校青年骨干教师培养计划项目(2020GGJS152) （2020GGJS152）
信阳师范大学"南湖学者奖励计划"青年项目 （）
信阳师范大学大学生科研基金项目 （）
