信阳师范学院学报（自然科学版）2024，Vol.37Issue(3)：399-404,6.DOI:10.3969/j.issn.1003-0972.2024.03.017
基于环介导等温扩增技术建立非洲猪瘟病毒的快速高灵敏度检测方法
Rapid High-sensitivity Detection of African Swine Fever Virus Based on Loop-mediated Isothermal Amplification
摘要
Abstract
In order to solve the problem that African swine fever seriously endangers China's pig industry,a loop-mediated isothermal amplification primer(LAMP)was designed according to the conserved sequence of African swine fever virus p72 gene by loop-mediated isothermal amplification technology,and then the primer was screened and the reaction conditions were optimized.The results showed that the optimized reaction system could detect African swine fever virus as low as 1 copy/pL at 45 min,and did not cross-react with other viral nucleic acid samples,the detection results could be judged with the naked eye,with good sensitivity and specificity.关键词
非洲猪瘟/环介导等温扩增技术(LAMP)/快速检测Key words
African swine fever/loop-mediated isothermal amplification(LAMP)/repaid detection method分类
农业科技引用本文复制引用
张朋朋,黄月茹,牛佳,解秀杰,伍文艳,程晓芳,李岑岑,徐海霞,徐永杰..基于环介导等温扩增技术建立非洲猪瘟病毒的快速高灵敏度检测方法[J].信阳师范学院学报（自然科学版）,2024,37(3):399-404,6.基金项目
国家自然科学基金项目(31601167) （31601167）
河南省重大科技专项项目(201300111200) （201300111200）
河南省高等学校重点科研项目(21A230017) （21A230017）
