烟草科技2024，Vol.57Issue(5)：35-40,6.
烟草粉螟性信息素结合蛋白EeluPBP的表达及结合特性
Expression and binding properties of pheromone binding protein EeluPBP in Ephestia elutella
摘要
Abstract
To understand the sex pheromone perception mechanisms in Ephestia elutella,a pheromone binding protein(EeluPBP)from Ephestia elutella was expressed in Escherichia coli in vitro.The binding capacity of EeluPBP protein with the two sex pheromones of E.elutella,(Z,E)-9,12-tetradecadien-1-ol and(Z,E)-9,12-tetradecadien-1-ol acetate,was evaluated by a fluorescence competitive binding assay.The results showed that:1)EeluPBP protein was successfully expressed in E.coli with a molecular weight of approximately 15 kDa.2)The binding dissociation constant Kd value of EeluPBP protein with fluorescent probe 1-NPN was 0.83 μmol/L,indicating that the EeluPBP protein expressed and purified in vitro had good binding activities.3)The recombinant EeluPBP protein had a strong binding capacity with(Z,E)-9,12-tetradecadien-1-ol with a Ki value of 3.26 μmol/L,indicating that the pheromone binding protein EeluPBP played an important role in the perception of sex pheromone in E.elutella.关键词
烟草粉螟/性信息素结合蛋白/荧光竞争结合试验Key words
Ephestia elutella/Pheromone binding protein/Fluorescence competitive binding assay分类
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王桂瑶,王建伟,刘丽,许强,李秋英,朱晋,杨萌萌,王永胜,周汉平,刘英杰..烟草粉螟性信息素结合蛋白EeluPBP的表达及结合特性[J].烟草科技,2024,57(5):35-40,6.基金项目
中国烟草总公司重点研发项目"基于替代磷化铝熏蒸的仓储害虫生态防控技术体系构建及应用"(110202202029) （110202202029）
郑州烟草研究院青年人才托举工程项目"烟草粉螟识别性信息素的分子机制研究"(122020CR0240) （122020CR0240）
江苏中烟工业有限责任公司项目"S-烯虫酯控制烟草仓贮害虫综合配套技术研究及应用"(H202223) （H202223）
河南省科技攻关项目"基于知识图谱的烟草病虫害专家系统构建技术研究"(232102210073) （232102210073）
广东中烟工业有限责任公司项目"库存偏紧核心产区原料醇化周期研究"(粤烟工科任[2021]-028号). （粤烟工科任[2021]-028号）
