烟草科技2024，Vol.57Issue(5)：41-47,7.
烟草靶斑病菌LFD-RAA快速检测方法的建立
A rapid LFD-RAA detection method for tobacco target spot pathogen
摘要
Abstract
To rapidly and effectively detect the tobacco target spot pathogen,the conserved region of the beta-tubulin gene sequence of the pathogen was targeted and the recombinase aided amplification(RAA)primers were designed.Lateral flow chromatographic strips were used to establish and further optimize a rapid detection system.The results showed that the developed RAA amplification could be completed within 30 minutes at a constant temperature of 39 ℃ and had high specificity with a detection limit of 100 fg/μL.Its sensitivity was comparable to that of conventional PCR detections.The optimal amplification time and temperature of the optimized detection system were 20-35 min and 31-39 ℃.This LFD-RAA detection system could be applied for rapid detection of the pathogen of tobacco target spot on tobacco plants.关键词
烟草靶斑病/重组酶介导扩增技术/检测Key words
Tobacco target spot/Recombinase aided amplification/Detection分类
农业科技引用本文复制引用
肖艳松,李思军,吴文信,刘天波,周向平,周陆苏,钟杰..烟草靶斑病菌LFD-RAA快速检测方法的建立[J].烟草科技,2024,57(5):41-47,7.基金项目
湖南省郴州市烟草公司烟草农业科技创新项目"郴州烟区烟草靶斑病和野火病病原微生物组解析及早期流行诊断"(CZYC2022JS03)、"郴州雪茄烟霉变机制与绿色防控技术研究"(CZYC2023JS05) （CZYC2022JS03）
中国烟草总公司湖南省公司科技重点项目"湖南烟草靶斑病成灾机制与绿色防控技术研究"(HN2021KJ01). （HN2021KJ01）
