烟草溃疡病病原鉴定及生物学特性分析OA北大核心CSTPCD
Identification and biological characterization of tobacco stalk canker pathogen
2022-2023年在湖南省永州市的烟田烟株上发现类似烟草溃疡病的茎部病害.为了明确引起该病害的病原种类及生物学特性,利用组织分离法对感病烟株茎秆进行分离纯化、形态学观察和柯赫氏法则验证,并对其生物学特性进行研究,同时进行形态学及rDNA-ITS序列进化分析发现,该病害的病原为葡萄座腔菌(Botryosphaeria dothidea).生物学特性分析表明,葡萄座腔菌的菌丝生长最适温度为30 ℃,最适pH值为7,最适碳源和氮源分别为葡萄糖和牛肉膏.这是在湖南产烟区首次被报道的由Botryosphaeria dothidea引起的烟草溃疡病.
From 2022 to 2023 crop year,a stalk disease resembling tobacco canker was observed on tobacco plants in Yongzhou County of Hunan Province.In order to identify the pathogen and clarify the biological characteristics of the disease,a tissue separation method was used to isolate and purify the pathogen from diseased stalks,then morphological observation and verification of Koch's postulates were carried out.The biological characteristics of the pathogen were investigated.Morphological and phylogenetic analyses of the rDNA-ITS sequence confirmed the pathogen as Botryosphaeria dothidea.Investigation into the biological characteristics of Botryosphaeria dothidea revealed an optimal mycelial growth temperature of 30 ℃,pH of 7,with glucose and bovine extract identified as the preferred carbon and nitrogen sources,respectively.This study represents the first report of tobacco canker caused by Botryosphaeria dothidea in Hunan Province.
钟杰;刘天波;邱泽澜;刘仕达;杨志娟;苏家恩;周彦夷;肖艳松;王迎;周向平
湖南农业大学植物保护学院,长沙市芙蓉区农大路1号 410128湖南省烟草科学研究所,长沙市暮云镇暮云新村 410010云南省烟草公司大理州公司,云南省大理州大理市下关镇鹤庆路71号 671000湖南省烟草公司郴州市公司桂阳县公司,湖南省郴州市桂阳县龙潭街道蔡伦北路42号 424400湖南省烟草公司永州市公司,湖南省永州市冷水滩区珍珠北路69号 425000
植物保护学
烟草病原菌葡萄座腔菌茎溃疡病生物学特性
TobaccoPathogenBotryosphaeria dothideaStalk cankerBiological characteristic
《烟草科技》 2024 (005)
58-63 / 6
湖南省郴州市烟草公司烟草农业科技创新项目"郴州烟区烟草靶斑病和野火病病原微生物组解析及早期流行诊断"(CZYC2022JS03);中国烟草总公司湖南省公司科技重点项目"湖南烟草靶斑病成灾机制与绿色防控技术研究"(HN2021KJ01);云南省烟草公司大理州公司科技计划一般项目"基于传毒介体控制与免疫诱抗的烟草番茄斑萎病生物防治研究与应用"(DLYC2022003).
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