烟草科技2024，Vol.57Issue(5)：58-63,6.
烟草溃疡病病原鉴定及生物学特性分析
Identification and biological characterization of tobacco stalk canker pathogen
摘要
Abstract
From 2022 to 2023 crop year,a stalk disease resembling tobacco canker was observed on tobacco plants in Yongzhou County of Hunan Province.In order to identify the pathogen and clarify the biological characteristics of the disease,a tissue separation method was used to isolate and purify the pathogen from diseased stalks,then morphological observation and verification of Koch's postulates were carried out.The biological characteristics of the pathogen were investigated.Morphological and phylogenetic analyses of the rDNA-ITS sequence confirmed the pathogen as Botryosphaeria dothidea.Investigation into the biological characteristics of Botryosphaeria dothidea revealed an optimal mycelial growth temperature of 30 ℃,pH of 7,with glucose and bovine extract identified as the preferred carbon and nitrogen sources,respectively.This study represents the first report of tobacco canker caused by Botryosphaeria dothidea in Hunan Province.关键词
烟草/病原菌/葡萄座腔菌/茎溃疡病/生物学特性Key words
Tobacco/Pathogen/Botryosphaeria dothidea/Stalk canker/Biological characteristic分类
农业科技引用本文复制引用
钟杰,刘天波,邱泽澜,刘仕达,杨志娟,苏家恩,周彦夷,肖艳松,王迎,周向平..烟草溃疡病病原鉴定及生物学特性分析[J].烟草科技,2024,57(5):58-63,6.基金项目
湖南省郴州市烟草公司烟草农业科技创新项目"郴州烟区烟草靶斑病和野火病病原微生物组解析及早期流行诊断"(CZYC2022JS03) （CZYC2022JS03）
中国烟草总公司湖南省公司科技重点项目"湖南烟草靶斑病成灾机制与绿色防控技术研究"(HN2021KJ01) （HN2021KJ01）
云南省烟草公司大理州公司科技计划一般项目"基于传毒介体控制与免疫诱抗的烟草番茄斑萎病生物防治研究与应用"(DLYC2022003). （DLYC2022003）
