Abstract
Objective To explore the clinical diagnostic value of microRNA-370-3p(miR-370-3p)and low density lipo-protein receptor-related protein 6(LRP6)in pregnant women with fetal growth restriction(FGR).Methods From June 2020 to June 2022,96 pregnant women who were diagnosed as FGR in our hospital were selected as observation group,and 96 healthy pregnant women examined in our hospital were selected as control group.And then,the gestational weeks of deliv-ery,1 min Apgar score,5 min Apgar score,newborn weight and placental quality of the two groups were recorded.Accord-ing to the standards of the American College of Obstetrics and Gynecology(ACOG),the observation group were divided into FGR group and severe FGR group.The expression levels of serum miR-370-3p and LRP6 mRNA were detected by qRT-PCR;the correlation between serum miR-370-3p and LRP6 mRNA levels and gestational weeks of delivery,1 min Apgar score,5 min Apgar score,newborn weight and placental quality was analyzed by Pearson method;and the diagnostic value of miR-370-3p and LRP6 mRNA for FGR was evaluated by ROC curve.Results There was statistically significant difference in the proportion of age,gestational weeks of delivery,and whether or not the first delivery between the two groups(P＜0.05);the miR-370-3p of the observation group was obviously higher than that of the control group,and LRP6 was obvious-ly lower than that of the control group(P＜0.05);the miR-370-3p of the severe FGR group was obviously higher than that of the FGR group,and the LRP6 was obviously lower than that of the FGR group(P＜0.05);there was statistically significant difference in terms of newborn weight,1 minute Apgar score,5 minute Apgar score,and placental quality between the con-trol group and the observation group(P＜0.05);there was negative correlation between miR-370-3p and LRP6(r=-0.692,P＜0.05),the miR-370-3p was negatively associated with gestational weeks of delivery,newborn weight,1 min Apgar score,5 min Apgar score,and placental quality(r=-0.401,-0.382,-0.425,-0.484,-0.504,all P＜0.05),the LRP 6 was positively correlated with gestational weeks of delivery,neonatal weight,1 min Apgar score,5 min Apgar score,and placen-tal quality(r=0.306,0.412,0.512,0.612,and 0.419,allP＜0.05);the ROC curve showed that the AUC of miR-370-3p for FGR diagnosis was 0.877(95％CI:0.821-0.919)with a cutoff value of 1.40,and its sensitivity and specificity were 67.71％and 93.75％,respectively;the AUC of LRP6 for FGR diagnosis was 0.838(95％CI:0.778-0.887),with a cutoff value of 0.83,and its sensitivity and specificity were 84.37％and 71.87％,respectively;and the AUC of FGR di-agnosis by the combination of the two was 0.923(95％CI:0.875-0.956),which was obviously higher than that diagnosis by each of the two alone(Zcombination vs miR-370-3P=2.811,P=0.005;Zcombination vs LRP6=3.372,P=0.001),and its sensitivity and specificity were 85.42％and 87.50％,respectively.Conclusion Serum miR-370-3p is highly expressed and LRP6 is lowly expressed in FGR patients,the combination of the two has certain diagnostic value for FGR.关键词
微小RNA-370-3p/低密度脂蛋白受体相关蛋白6/胎儿生长受限/诊断Key words
microRNA-370-3p/low density lipoprotein receptor-related protein 6(LRP6)/fetal growth restriction/diagnosis分类
医药卫生
