疑难病杂志2024，Vol.23Issue(7)：823-827,833,6.DOI:10.3969/j.issn.1671-6450.2024.07.012
阿尔茨海默病患者血清miR-132-3p、SMAD2水平及其临床意义
The expression levels and clinical significance of serum miR-132-3p and SMAD2 in patients with Alzheimer's disease
摘要
Abstract
Objective To explore the prognostic value of microRNA-132-3p(miR-132-3p)and SMAD2 in patients with Alzheimer's disease(AD).Methods From January 2020 to December 2022,82 AD patients treated in Department of Neurology,Jiamusi Central Hospital were collected as research subjects,according to the prognosis after 6 months,they were grouped into a good prognosis group(39 cases)and a poor prognosis group(43 cases),the baseline data of the pa-tient were recorded.Real-time fluorescence quantitative PCR(qRT-PCR)method was applied to detect serum levels of miR-132-3p,enzyme-linked immunosorbent assay was applied to detect serum levels of SMAD2;Pearson and Spearman correla-tions were applied to analyze and test the correlation between miR-132-3p,SMAD2 levels and baseline data in AD patients with poor prognosis;ROC curve was applied to analyze the predictive value of serum miR-132-3p and SMAD2 levels for poor prognosis in AD patients;Logistic multiple factor regression was applied to analyze the influencing factors of poor prognosis in AD patients.Results Serum levels of SMAD2 and the proportion of mid/late stage AD,CRP,and Hey in the poor prognosis group were higher than those in the good prognosis group,while serum levels of miR-132-3p,and HDL-C were lower than those in the good prognosis group(t/x2=7.362,5598,14.021,12.593,7.145,6.655,P＜0.05).MiR-132-3p in AD patients with poor prognosis was negatively correlated with AD staging,CRP,Hcy,and positively correlated with HDL-C(r=-0.513,-0.492,-0.507,0.485,P＜0.001);SMAD2 in AD patients with poor prognosis was positively correlated with AD stage,CRP,Hcy,and negatively correlated with HDL-C(r=0.504,0.527,0.510,-0.496,P＜0.001).The AUC of ser-um miR-132-3p and SMAD2 alone and in combination for predicting poor prognosis in AD patients was 0.828,0.835,and 0.910,the combined predictive value of the two is greater than that of the two alone(Z=2.148,1.964,P=0.032,0.046).AD staging was an independent risk factor for poor prognosis in AD patients,and miR-132-3p were protective factors for poor prognosis in AD patients,SMAD2 is an independent risk factor for poor prognosis in AD patients(OR:95％CI=2.978(1.609-5511),P=0.001;OR:95％CI=0.828(0.722-0.950),P=0.007;OR:95％CI=2.826(1.776-4.497),P＜0.001).Conclu-sion The expression of miR-132-3p is reduced in AD patients with poor prognosis,the expression of SMAD2 is increased,and they may become serum markers of poor prognosis in AD patients,which can to some extent predict the development of the disease in AD patients.关键词
阿尔茨海默病/微小RNA-132/SMAD2/预后Key words
Alzheimer's disease/Micro RNA-132/SMAD2/Prognosis分类
医药卫生引用本文复制引用
褚春沐,隗永健,贾丽娜,杨思远,张洁..阿尔茨海默病患者血清miR-132-3p、SMAD2水平及其临床意义[J].疑难病杂志,2024,23(7):823-827,833,6.基金项目
黑龙江省卫生健康委科研课题(2022-159) Research Project of Heilongjiang Provincial Health Commission(2022-159) （2022-159）
