云南化工2024，Vol.51Issue(7)：118-121,4.DOI:10.3969/j.issn.1004-275X.2024.07.27
超高效液相色谱-串联质谱法测定枸杞提取物中的甜菜碱
Determination of Betaine in Lycium Barbarum Extract by Ultra Performance Liquid Chromatography-tandem Mass Spectrometry
李洁莹 1钟建权 1彭静雅 2蔡杏宜 2林钦恒 2潘云山2
作者信息
- 1. 广东省绿色产品认证检测中心有限公司,广东 广州 510440
- 2. 广东省科学院测试分析研究所 (中国广州分析测试中心),广东省化学测量与应急检测技术重点实验室,广东省保健食品功效成分检测与风险物质快速筛查工程技术研究中心,广东 广州 510070
- 折叠
摘要
Abstract
The experiment aimed to establish a method for the determination of betaine in Lycium barbarum extract by ultra-high performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS).Betaine in Lycium barbarum extrac sample was extracted ultrasonically with 50%methanol,diluted with water,and separated on an Agilent InfinityLab Poroshell 120 HILIC(ϕ2.1 mm×150 mm,2.7 μm)column with gradient elution using the mobile phases consisting of 5 mmoL/L ammonium acetate aqueous solution and acetonitrile.Quantitative analysis was carried out by electrospray ionizationsource in positive ion mode under multi reaction monitoring(MRM)mode,using external standard method.The results showed good linearity for betaine in the concentration range of 10-500 ng/mL,with a correlation coefficient(r2)of 0.9994.The limits of detection(LOD)and quantitation(LOQ)were 0.12 mg/kg and 0.35 mg/kg,respectively.The spiked experiments were performed at three concentration levels,and the average recovery rates for betaine ranged from81.5%to 102.7%,with relative standard deviations(RSDs)of 1.0%-7.5%(n=6).The study indicates that the method is feasible,simple,rapid,and exhibits high sensitivity,high accuracy,and good reproducibility,making it effective for detecting the concentration of betaine in Lycium barbarum extract.关键词
超高效液相色谱-串联质谱法/甜菜碱/枸杞提取物Key words
Ultra Performance Liquid Chromatography-tandem Mass Spectrometry/Betaine/Lycium Barbarum Extract分类
化学引用本文复制引用
李洁莹,钟建权,彭静雅,蔡杏宜,林钦恒,潘云山..超高效液相色谱-串联质谱法测定枸杞提取物中的甜菜碱[J].云南化工,2024,51(7):118-121,4.
