烟台大学学报（自然科学与工程版）2024，Vol.37Issue(3)：281-292,12.DOI:10.13951/j.cnki.37-1213/n.230407
结合态PC645组分的分离纯化和性质
Isolation and Characterization of PC645 in Binding State from Chroomonas placoidea
摘要
Abstract
A Chl-protein band named Band Ⅲ containing phycocyanin PC645 in binding state is isolated from Chroomonas placoidea by using the method of sucrose density gradient centrifugation.The results of ammonium sul-fate fractionation show that 50％ammonium sulfate precipitation can remove most of the Chl-protein complexes in Band Ⅲ and effectively avoid the loss of PC645.Spectral and peptide analysis demonstrate that the subunit type of the most abundant band Band Ⅰ is β1,indicating that the α1α2β1β1 heterodimer form predominated in PC645 is mainly free in the thylakoid lumen.While,the heterodimer containing 2 subunit enriched in Band Ⅲ is probablyα1α2β1 β2,which exhibits higher hydrophobicity and therefore more susceptible to be salted out.Band Ⅲ after am-monium sulfate fractionation is subject to hydrophobic chromatography.The eluted fraction 1 with relatively lower hydrophobicity contains both PC645 and Chl a-protein.The eluted fraction 4 with relatively highest hydrophobicity is recognized as fully purified PC645 containing almost no chlorophyll,and most likely the PC645 component of Band Ⅲ in binding state to Chl-protein.These results lay a foundation for structure and interaction studies between the two sets of light-harvesting systems in cryptophytic algae in the future.关键词
蓝隐藻/PC645-Chl-蛋白复合物/蔗糖密度梯度离心/硫酸铵分级沉淀/疏水层析Key words
Chroomonas placoidea/PC645-chlorophyll-protein complex/sucrose density gradient centrifugation/ammonium sulfate fractionation/hydrophobic chromatography分类
农业科技引用本文复制引用
王宇涵,任靖文,陈敏..结合态PC645组分的分离纯化和性质[J].烟台大学学报（自然科学与工程版）,2024,37(3):281-292,12.基金项目
山东省自然科学基金资助项目(ZR2018LD009). （ZR2018LD009）
