烟台大学学报（自然科学与工程版）2024，Vol.37Issue(3)：293-298,6.DOI:10.13951/j.cnki.37-1213/n.230519
栉孔扇贝Tssk2基因的序列特征及表达分析
Sequence Characteristics and Expression Analysis of Tssk2 Gene in Chlamys farreri
摘要
Abstract
In order to study the sequence and expression pattern of testis-specific serine/threonine-protein kinase 2 gene(Tssk2)in Chlamys farreri,the sequence characteristics,tissue expression and gonadal cycle expression of Tssk2 gene are studied by qRT-PCR and bioinformatics analysis.The results are as follows:The CDS(coding se-quences)of Tssk2 is 723 bp long and encodes 240 amino acids.Phylogenetic analysis shows that the evolutionary status of Tssk2 protein in C.farreri is consistent with its biological classification status.Tssk2 possesses the S_TKc conserved domain of the TSSKs,including the serine/threonine protein kinase active region(Ⅵb region)and ATP-binding region,but the ATP-binding region lacks the Ⅰ subdomain.The expression of Tssk2 gene in various tissues and gonadal development cycles of C.farreri show that Tssk2 expression is the highest in the sperms,especially in the sperms at maturity,which shows that this gene may play a key role in mature testes and/or is relates to sperm maturation.关键词
栉孔扇贝/Tssk2/序列特征/表达Key words
Chlamys farreri/Tssk2/sequence feature/expression分类
农业科技引用本文复制引用
邢妍,王春雨,罗冬艳,刘晓玲..栉孔扇贝Tssk2基因的序列特征及表达分析[J].烟台大学学报（自然科学与工程版）,2024,37(3):293-298,6.基金项目
山东省自然科学基金资助项目(ZR2022MC126) （ZR2022MC126）
山东省贝类产业技术体系项目(SDAIT-14-05). （SDAIT-14-05）
