寒邪对大鼠腰椎间盘突出症机体影响的实验研究OA
Experimental Study on the Effect of Cold Pathogen on Lumbar Disc Herniation in Rats
目的 探讨寒邪在大鼠腰椎间盘突出症模型中的影响.方法 将30只SD级大鼠按照随机数字表法随机分为实验组、对照组和空白组,各10例.实验组和对照组通过自体髓核移植至L4、L5神经根建立大鼠腰椎间盘突出症模型,空白组不做处理,实验组和空白组连续7 d每天12:00-16:00将大鼠置于温度为(4±0.5)℃、湿度为50％±2％的人工气候箱中饲养4 h,其余时间按常规室温饲养.对照组放置于(25±0.5)℃、湿度为50％±2％的常温中标准化饲养1周.观察三组大鼠生存质量、髓核中PLA2含量、血清炎症因子TNF-α、IL-6含量变化、椎间盘病理切片情况.结果 造模完成后,三组大鼠全部存活,无死亡、瘫痪、感染案例,但于实验第3天,三组大鼠均有明显症状,神经功能评分均在3级以上.实验第7、14天,实验组神经功能评分均高于对照组和空白组(P＜0.05);实验组实验第14天与第7天神经功能评分比较,差异无统计学意义(P＞0.05);而对照组、空白组实验第14天与第7天神经功能评分,差异有统计学意义(P＜0.05).实验第14天血清TNF-α、IL-6水平均低于实验第7天,但实验组实验第7、14天血清TNF-α、IL-6水平高于对照组和空白组(P＜0.05).三组实验第14天大鼠髓核中PLA2含量均低于实验第7天,但实验组实验第7、14天大鼠髓核中PLA2含量高于对照组和空白组(P＜0.05).结论 寒邪作用于大鼠腰椎间盘突出症模型能降低大鼠生存质量,提高髓核中PLA2含量及血清炎症因子TNF-α、IL-6表达,为腰椎间盘突出症的重要发病因素.
Objective To investigate the effect of cold pathogen on rat model of lumbar disc herniation.Methods Thirty SD rats were randomly divided into experimental group,control group and blank group according to the random number table method,with 10 rats in each group.The experimental group and the control group were transplanted with autologous nucleus pulposus to L4 and L5 nerve roots to establish a rat model of lumbar disc herniation.The blank group was not treated.The experimental group and the blank group were placed at 12:00-16:00 every day for 7 consecutive days.The rats were raised in an artificial climate box with a temperature of(4±0.5)℃ and a humidity of 50％±2％for 4 h,and the rest of the time was raised at room temperature.The control group was placed in a room temperature of(25±0.5)℃ and a humidity of 50％±2％for standardized feeding for 1 week.The quality of life,the content of PLA2 in nucleus pulposus,the content of serum inflammatory factors TNF-α and IL-6,and the pathological section of intervertebral disc were observed in the three groups.Results After the completion of the modeling,all the three groups of rats survived without death,paralysis,and infection.On the third day of the experiment,the three groups of rats had obvious symptoms,and the neurological function scores were all above grade 3.On the 7th and 14th day of the experiment,the neurological function scores of the experimental group were higher than those of the control group and the blank group(P＜0.05),there was no significant difference in neurological function score between the 14th day and the 7th day in the experimental group(P＞0.05),while the neurological function scores of the control group and the blank group on the 14th and 7th days of the experiment were statistically significant(P＜0.05).The levels of serum TNF-α and IL-6 on the 14th day of the experiment were lower than those on the 7th day of the experiment,but the levels of serum TNF-α and IL-6 on the 7th and 14th days of the experiment in the experimental group were higher than those in the control group and the blank group(P＜0.05).The content of PLA2 in the nucleus pulposus of rats on the 14th day of the three groups was lower than that on the 7th day of the experiment,but the content of PLA2 in the nucleus pulposus of rats on the 7th and 14th days of the experiment in the experimental group was higher than that in the control group and the blank group(P＜0.05).Conclusion The cold pathogen acting on the rat model of lumbar disc herniation can reduce the quality of life of rats,increase the content of PLA2 in the nucleus pulposus and the expression of serum inflammatory factors TNF-α and IL-6,which is an important pathogenic factor of lumbar disc herniation.
徐丽;李孝林;张朝驹;彭昌华;陈洪卫;吴磊磊;何川
荆州市中医药研究所/荆州市中医医院骨科,湖北荆州 434000长江大学医学院,湖北 荆州 434000
临床医学
腰椎间盘突出症寒邪TNF-αIL-6PLA2
Lumbar disc herniationCold pathogenTNF-αIL-6PLA2
《医学信息》 2024 (014)
90-95 / 6
1.全国名老中医专家传承工作室建设项目(编号:国中医药人教函[2022]75号);2.湖北省中医药管理局中医药科研面上项目(编号:ZY2023M051);3.荆州市中医药研究所自主选题项目(编号:ZZXT2021Y05)
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