空军军医大学学报2024，Vol.45Issue(7)：763-768,6.DOI:10.13276/j.issn.2097-1656.2024.07.007
肝癌阿帕替尼耐药细胞株的构建及耐药基因研究
Construction of apatinib-resistant cell lines in hepatocellular carcinoma and study of resistant genes
摘要
Abstract
Objective To explore the potential resistance mechanism of hepatocellular carcinoma cells to apatinib.Methods Human SNU-739/SNU-878 hepatocellular carcinoma cell lines were selected to construct apatinib-resistant cell lines.Antimicrobial susceptibility testing was carried out on parental and drug-resistant cells by cell viability detection and plate clone formation assay,the gene expression profiles of parental and drug-resistant cells were detected by RNA chip technology,and the key genes mediating apatinib resistance were screened out according to the expression differences.qRT-PCR and Western blotting were used to verify the key genes,and the effect of stable overexpression of key genes on cell drug resistance was detected by antimicrobial susceptibility testing.Results The cell viability and number of clones formed in apatinib-resistant cell lines were significantly higher than those in parental cells(P＜0.05).A key gene,carbamoyl phosphate synthetase 1(CPS1),was screened out,and its mRNA and protein levels were significantly down-regulated in drug-resistant cell lines(P＜0.05).Overexpression of CPS1 significantly reduced the viability and clonal formation ability of hepatocellular carcinoma cells(P＜0.05).Conclusion CPS1 may be a key gene that mediates apatinib resistance in hepatocellular carcinoma cells.关键词
肝癌/阿帕替尼/耐药/氨基甲酰磷酸合成酶1Key words
hepatocellular carcinoma/apatinib/drug resistance/carbamoyl phosphate synthetase 1分类
医药卫生引用本文复制引用
博伦,王祥旭,纪洪辰,艾丽萍,白引苗,刘洋,张红梅..肝癌阿帕替尼耐药细胞株的构建及耐药基因研究[J].空军军医大学学报,2024,45(7):763-768,6.基金项目
陕西省自然科学基础研究计划重点项目(2021JZ-35) （2021JZ-35）
空军军医大学西京医院临床研究专项-重点项目(XJZT24LZ15) （XJZT24LZ15）
