连朴饮基准样品高效液相色谱指纹图谱建立及化学成分鉴定OA北大核心CSTPCD

High performance liquid chromatography(HPLC)technique was used to establish the fingerprints of the benchmark samples of Lianpo Decoction(LPD).The experiment was performed on a Welch Ultimate®XB-C18(250 mm×4.6 mm,5 μm)column with methanol-0.1％phosphoric acid solution as the mobile phase in a gradient elution with the detection wavelength of 254 nm,the column temperature of 30 ℃,the volume flow rate of 1.0 mL/min,and the injection volume of 10 μL.The quality of different batches of LPD was evaluated by similarity evaluation,cluster analysis(CA),principal component analysis(PCA)and orthogonal partial least squares-discriminant analysis(OPLS-DA).Ultra performance liquid chromatography-quadrupole-electrostatic field orbitrap high resolution mass spectrometry(UPLC-Q-Orbitrap HRMS)was applied to identify the chemical constituents in LPD.The results showed that the similarity between the fingerprints of the benchmark samples of 15 batches of LPD and the control fingerprints was higher than 0.9,and the methodological validation of the HPLC fingerprints of the benchmark samples of LPD was good.Twenty-three common peaks were identified,and eight components were recognized,which were geniposide at peak 3,epiberine at peak 7,coptis hydrochloride at peak 8,berberine hydrochloride at peak 11,palmatine hydrochloride at peak 12,genistein at peak 19,honokiol at peak 21,magnolol at peak 22,respectively.The 15 batches of benchmark samples were classified into three categories by two chemical pattern recognition methods,cluster analysis and principal component analysis,and the results corroborated each other.Principal components 1 to 4 were the main factors affecting their quality evaluation,and a total of 12 differential markers were identified by OPLS-DA.Ninety-one compounds were identified in LPD,which mainly included cyclic enol ether terpenes,organic acids,flavonoid glycosides,flavonoids and isoflavonoids,alkaloids,lignans and amino acids.The HPLC fingerprints of the baseline samples of LPD were simple,stable and reproducible,and combined with the mass spectrometry method,it can provide a reference for the subsequent formulation development and quality control studies.