连朴饮基准样品高效液相色谱指纹图谱建立及化学成分鉴定OA北大核心CSTPCD
Establishment of High Performance Liquid Chromatographic Fingerprints and Identification of Chemical Constituents in Benchmark Samples of Lianpo Decoction
采用高效液相色谱(HPLC)技术建立连朴饮(LPD)基准样品的指纹图谱,并对其中的化学成分进行分析.该实验使用Welch Ultimate®XB-C18(250 mm×4.6 mm,5 μm)色谱柱,以甲醇-0.1％磷酸溶液作为流动相,梯度洗脱,检测波长为254 nm,柱温为30 ℃,体积流量为1.0 mL/min,进样量为10 µL.通过相似度评价、聚类分析(CA)、主成分分析(PCA)和正交偏最小二乘法-判别分析(OPLS-DA)对不同批次连…查看全部>>
High performance liquid chromatography(HPLC)technique was used to establish the fingerprints of the benchmark samples of Lianpo Decoction(LPD).The experiment was performed on a Welch Ultimate®XB-C18(250 mm×4.6 mm,5 μm)column with methanol-0.1％phosphoric acid solution as the mobile phase in a gradient elution with the detection wavelength of 254 nm,the column temperature of 30 ℃,the volume flow rate of 1.0 mL/min,and the injection volume of 10 μL.The quality …查看全部>>
王婕;王书航;周绍岩;梁潇云;徐可进
长春中医药大学药学院,长春 130117长春中医药大学药学院,长春 130117长春中医药大学药学院,长春 130117长春中医药大学药学院,长春 130117长春中医药大学药学院,长春 130117
化学
连朴饮聚类分析高效液相色谱指纹图谱超高效液相色谱-四极杆-静电场轨道阱高分辨质谱主成分分析正交偏最小二乘法-判别分析
Lianpo decoctionCluster analysisHigh performance liquid chromatography fingerprintingUPLC-Q-Orbitrap HRMSPrincipal component analysisOrthogonal partial least squares-discriminant analysis
《应用化学》 2024 (6)
813-829,17
吉林省创新能力建设项目(No.2021C002)、吉林省教育厅科学研究项目(No.JJKH20241093KJ)和重大疫情防治经典名方制剂储备库建设项目(No.吉中医药发[2021]11号)资助 Supported by Jilin Province Innovation Capacity Building Project(No.2021C002),Scientific Research Project of Jilin Provincial Department of Education(No.JJKH20241093KJ)and Construction Project of Reserve Bank of Classical Famous Formulas for Prevention and Control of Major Epidemics([2021]No.11)
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