应用化学2024，Vol.41Issue(6)：813-829,17.DOI:10.19894/j.issn.1000-0518.230351
连朴饮基准样品高效液相色谱指纹图谱建立及化学成分鉴定
Establishment of High Performance Liquid Chromatographic Fingerprints and Identification of Chemical Constituents in Benchmark Samples of Lianpo Decoction
摘要
Abstract
High performance liquid chromatography(HPLC)technique was used to establish the fingerprints of the benchmark samples of Lianpo Decoction(LPD).The experiment was performed on a Welch Ultimate®XB-C18(250 mm×4.6 mm,5 μm)column with methanol-0.1％phosphoric acid solution as the mobile phase in a gradient elution with the detection wavelength of 254 nm,the column temperature of 30 ℃,the volume flow rate of 1.0 mL/min,and the injection volume of 10 μL.The quality of different batches of LPD was evaluated by similarity evaluation,cluster analysis(CA),principal component analysis(PCA)and orthogonal partial least squares-discriminant analysis(OPLS-DA).Ultra performance liquid chromatography-quadrupole-electrostatic field orbitrap high resolution mass spectrometry(UPLC-Q-Orbitrap HRMS)was applied to identify the chemical constituents in LPD.The results showed that the similarity between the fingerprints of the benchmark samples of 15 batches of LPD and the control fingerprints was higher than 0.9,and the methodological validation of the HPLC fingerprints of the benchmark samples of LPD was good.Twenty-three common peaks were identified,and eight components were recognized,which were geniposide at peak 3,epiberine at peak 7,coptis hydrochloride at peak 8,berberine hydrochloride at peak 11,palmatine hydrochloride at peak 12,genistein at peak 19,honokiol at peak 21,magnolol at peak 22,respectively.The 15 batches of benchmark samples were classified into three categories by two chemical pattern recognition methods,cluster analysis and principal component analysis,and the results corroborated each other.Principal components 1 to 4 were the main factors affecting their quality evaluation,and a total of 12 differential markers were identified by OPLS-DA.Ninety-one compounds were identified in LPD,which mainly included cyclic enol ether terpenes,organic acids,flavonoid glycosides,flavonoids and isoflavonoids,alkaloids,lignans and amino acids.The HPLC fingerprints of the baseline samples of LPD were simple,stable and reproducible,and combined with the mass spectrometry method,it can provide a reference for the subsequent formulation development and quality control studies.关键词
连朴饮/聚类分析/高效液相色谱指纹图谱/超高效液相色谱-四极杆-静电场轨道阱高分辨质谱/主成分分析/正交偏最小二乘法-判别分析Key words
Lianpo decoction/Cluster analysis/High performance liquid chromatography fingerprinting/UPLC-Q-Orbitrap HRMS/Principal component analysis/Orthogonal partial least squares-discriminant analysis分类
化学化工引用本文复制引用
王婕,王书航,周绍岩,梁潇云,徐可进..连朴饮基准样品高效液相色谱指纹图谱建立及化学成分鉴定[J].应用化学,2024,41(6):813-829,17.基金项目
吉林省创新能力建设项目(No.2021C002)、吉林省教育厅科学研究项目(No.JJKH20241093KJ)和重大疫情防治经典名方制剂储备库建设项目(No.吉中医药发[2021]11号)资助 Supported by Jilin Province Innovation Capacity Building Project(No.2021C002),Scientific Research Project of Jilin Provincial Department of Education(No.JJKH20241093KJ)and Construction Project of Reserve Bank of Classical Famous Formulas for Prevention and Control of Major Epidemics([2021]No.11) （No.2021C002）
