应用生态学报2024，Vol.35Issue(6)：1681-1688,8.DOI:10.13287/j.1001-9332.202406.011
亚热带典型杉木人工林根际土壤固氮菌丰度和群落结构
Diazotrophic abundance and community structure in rhizosphere soils of typical subtropical Cunninghamia lanceolata plantations
摘要
Abstract
Rhizosphere is a vital area for substance exchange and energy transfer between roots and soil microor-ganisms.Therefore,diazotrophs in the rhizosphere play a pivotal role in facilitating plant nitrogen acquisition.We investigated the variability in the abundance and community structure of soil diazotrophs and the influencing factors across rhizosphere soils of Cunninghamia lanceolata in three locations:Baisha State-owned Forest Farm in Longyan City(BS),Sanming Forest Ecosystem and Global Change Research Station(SM),and Wuyishan National Forest Park in Nanping City(WYS),located in the western region of Fujian Province,quantified the diazotrophic abun-dance by using real-time quantitative PCR,and assessed the community structure by high-throughput sequencing.The results showed that soil pH,C∶N ratio,and C∶(N∶P)stoichiometry in SM were notably lower compared to those in BS and WYS.In SM,the abundance of the nifH gene was 6.38×108 copies·g-1,significantly lower than 1.35×109 copies·g-1 in BS and 1.10×109 copies·g-1 in WYS.Additionally,α diversity index of diazotrophs was lower in SM compared to BS and WYS,while the community structure of diazotrophs in rhizosphere soils of BS and WYS was similar,which differed significantly from that in SM.The diazotrophic sequences in the three forest farms could be divided into 5 phylum,8 classes,15 orders,23 families and 33 genera,with Proteobacteria,α-proteobac-teria,and Bradyrhizobium as the dominant phylotypes.Soil pH,available phosphorus,NO3--N and C∶(N∶P)ratio were identified as significant factors influencing both the abundance and community structure of nifH genes,with soil pH performing the greatest.Taken together,there were spatial variations in the distribution of diazotrophic abundance and community structure in C.lanceolata rhizosphere soils,with soil pH as the primary driving factor.关键词
杉木人工林/固氮菌/群落结构/多样性/化学计量比Key words
Cunninghamia lanceolata/diazotroph/community structure/diversity/stoichiometry引用本文复制引用
韩风毅,张贻荣,王思荣,杨智杰,郑勇,邓米林,贺纪正,林永新..亚热带典型杉木人工林根际土壤固氮菌丰度和群落结构[J].应用生态学报,2024,35(6):1681-1688,8.基金项目
本文由国家自然科学基金项目(42077041,31971447)资助. （42077041,31971447）
