应用生态学报2024，Vol.35Issue(6)：1695-1704,10.DOI:10.13287/j.1001-9332.202406.032
蚂蚁筑巢对热带橡胶人工林土壤甲烷排放季节动态的影响
Effects of ant nesting on seasonal dynamics of soil CH4 emissions in a tropical rubber-plantation forest
摘要
Abstract
Ant nests can affect the process and seasonal dynamics of forest soil methane emissions through media-ting methane oxidation/reduction microorganisms and physicochemical environments.To explore the process and mechanism by which ant nests affect soil methane emissions from Hevea brasiliensis plantation in Xishuangbanna,we measured the seasonal dynamics of methane emissions from ant nest and non-nest soils by using static chamber-gas chromatography method,and analyzed the effect of ant nesting on the changes in functional microbial diversity,mi-crohabitats,and soil nutrients in the plantations.The results showed that:1)Ant nests significantly affected the mean annual soil methane emissions in tropical plantation.Methane emissions in ant nest were decreased by 59.9％than the non-nest soil.In the dry season,ant nest soil was a methane sink(-1.770 μg·m-2·h-1),which de-creased by 87.2％compared with the non-nest soil,while it was a methane source(0.703 μg·m-2·h-1)that in-creased by 152.7％in the wet season.2)Ant nesting affected methane emissions via changing soil temperature,hu-midity,carbon and nitrogen concentrations.In contrast to the control,the mean annual temperature,humidity,and carbon and nitrogen content increased by 4.9％-138.5％in ant nest soils,which explained 90.1％,97.3％,27.3％-90.0％of the variation in methane emissions,respectively.3)Ant nesting affected the emission dynamics through changing the diversity and community structure of methane functional microbe.Compared with the control,the average annual methanogen diversity(Ace,Chao1,Shannon,and Simpson indices)in the ant nest ranged from-9.9％to 61.2％,which were higher than those(-8.7％-31.2％)of the methane-oxidising bacterial commu-nities.The relative abundance fluctuations of methanogens and methanotrophic bacteria were 46.76％and-6.33％,respectively.The explaining rate of methanogen diversity to methane emissions(78.4％)was higher than that of oxidizing bacterial diversity(54.5％),the relative abundance explained by the dominant genus of methanogens was 68.9％.4)The structural equation model showed that methanogen diversity,methanotroph diversity,and soil mois-ture were the main factors controlling methane emissions,contributing 95.6％,95.0％,and 91.2％to the variations of emissions,respectively.The contribution(73.1％-87.7％)of soil temperature and carbon and nitrogen compo-nents to the emission dynamics was ranked the second.Our results suggest that ant nesting mediates the seasonal dynamics of soil methane emissions,primarily through changing the diversity of methane-function microorganisms and soil water conditions.The research results deepen the understanding of the mechanism of biological regulation of methane emission in tropical forest soil.关键词
蚁巢/甲烷排放/甲烷产生菌/甲烷氧化菌/热带橡胶林Key words
ant nesting/methane emission/methanogen/methanotroph/tropical rubber forest引用本文复制引用
王郑钧,杨胜秋,兰梦杰,王邵军,肖博,解玲玲,郭志鹏,郭晓飞,李瑞,罗双,夏佳慧..蚂蚁筑巢对热带橡胶人工林土壤甲烷排放季节动态的影响[J].应用生态学报,2024,35(6):1695-1704,10.基金项目
本文由国家自然科学基金项目(32271722,32060281)和云南省一流学科建设项目(云教发[2022]73号)资助. （32271722,32060281）
