现代畜牧科技Issue(7)：29-33,5.DOI:10.19369/j.cnki.2095-9737.2024.07.009
罗伊氏乳酸菌外囊泡对LPS诱导的奶牛子宫内膜上皮细胞炎症的影响
The Effect of Lactobacillus reuteri Extracellular Vesicles on LPS-induced Inflammation of Bovine Endometrial Epithelial Cells
张亮1
作者信息
- 1. 甘肃畜牧工程职业技术学院,甘肃 武威 733006
- 折叠
摘要
Abstract
The aim of this study was to investigate the effect of Lactobacillus reuteri extracellular vesicles on LPS-induced inflammation in bovine endometrial epithelial cells.The outer vesicles of lactic acid bacteria were extracted by ultracentrifugation and representation was characterized.The intake of BEEC was detected by PKH-26 labeled outer vesicles.RT-qPCR WB and ELISA were used to detect the effects of EVs on the levels of PCNA,TNF-α,IL-1β,IL-6,IL-10 and NF-κB in LPS-induced BEEC inflammatory response.The result display that BEEC could ingest the outer vesicles of lactic acid bacteria,and the outer vesicles could promote the proliferation of BEEC.In LPS-induced BEEC cell inflammation,EVs can effectively inhibit the release of TNF-α,IL-β,IL-6 and NF-κB phosphorylation levels,and can promote the release of IL-10.Lactobacillus reuteri outer vesicles could alleviate BEEC cell inflammation by regulating the release of inflammatory factors.关键词
益生菌/罗伊氏乳酸菌/外囊泡/炎性反应Key words
probiotic/Lactobacillus reuteri/extracellular vesicles/inflammatory reaction分类
农业科技引用本文复制引用
张亮..罗伊氏乳酸菌外囊泡对LPS诱导的奶牛子宫内膜上皮细胞炎症的影响[J].现代畜牧科技,2024,(7):29-33,5.
