姜黄素通过调控p53/SLC7A11/GPX4通路抑制脂多糖诱导的RAW264.7源性破骨细胞分化OA北大核心CSTPCD
Curcumin inhibits lipopolysaccharide-induced differentiation of RAW264.7 cell-derived osteoclasts through regulation of p53/SLC7A11/GPX4 pathway
目的:探讨姜黄素(Cur)对脂多糖(LPS)诱导的RAW264.7源性破骨细胞的作用及其机制.方法:用LPS诱导RAW264.7细胞建立破骨细胞模型.CCK-8法检测RAW264.7细胞活力;TRAP染色鉴定破骨细胞形成;TRAP活性测定检测破骨细胞活力;生化检测细胞活性氧(ROS)、Fe2+、谷胱甘肽(GSH)及丙二醛(MDA)水平;透射电镜观察细胞线粒体形态;RT-qPCR检测p53、溶质载体家族7成员11(SLC7A11)及谷胱甘肽过氧化物酶4(GPX4)的mRNA水平;Western blot检测p53、SLC7A11及GPX4蛋白水平.结果:LPS成功诱导RAW264.7细胞为破骨细胞.TRAP染色及TRAP活性结果显示,与LPS组相比,Cur组破骨细胞数量及TRAP活性呈剂量依赖式下降(P<0.01);与Con组相比,LPS+Erastin组TRAP活性显著升高(P<0.01),经Cur处理后,TRAP活性呈剂量依赖式下降(P<0.01).生化检测结果显示,与Con组比较,LPS+Erastin组ROS、Fe2+及MDA水平显著升高,GSH水平显著降低(P<0.01);与LPS+Erastin组相比,Cur组ROS、Fe2+及MDA水平呈剂量依赖式下降(P<0.01),GSH水平呈剂量依赖式上升(P<0.01).电镜结果显示,与LPS组比较,LPS+Erastin组细胞线粒体嵴减少,膜密度增加;经Cur处理后,细胞线粒体嵴明显增加,膜密度减小.RT-qPCR和Western blot结果显示,与Con组比较,LPS+Erastin组p53的mRNA和蛋白水平显著升高,SLC7A11和GPX4的mRNA和蛋白水平显著降低(P<0.01);经Cur处理后,p53的mRNA和蛋白水平显著降低,SLC7A11和GPX4的mRNA和蛋白水平显著升高(P<0.01).结论:Cur可抑制LPS诱导的RAW264.7源性破骨细胞分化,其机制可能与p53/SLC7A11/GPX4信号通路被抑制有关.
AIM:The study aimed to explore the effect of curcumin(Cur)on lipopolysaccharide(LPS)-in-duced RAW264.7 cell-derived osteoclasts,together with its underlying mechanism.METHODS:An osteoclast model was established by treating RAW264.7 cells with LPS.The viability of the cells was assessed by CCK-8 assays and osteo-clast formation was evaluated by tartrate-resistant acid phosphatase(TRAP)activity.The levels of reactive oxygen species(ROS),Fe2+,glutathione(GSH),and malondialdehyde(MDA)were examined by biochemical assays.Mitochondrial morphology was assessed by transmission electron microscopy.The mRNA and protein levels of p53,glutathione peroxi-dase 4(GPX4),and solute carrier family 7 member 11(SLC7A11)were determined by RT-qPCR and Western blot,re-spectively.RESULTS:Treatment with LPS successfully induced osteoclasts formation in RAW264.7 cells.The TRAP results showed that compared with the LPS-treated group,the number of osteoclasts and TRAP activity in the curcumin-treated group decreased dose-dependently(P<0.01).Compared with the control group,the LPS+Erastin group showed significantly increased TRAP activity(P<0.01),while after curcumin treatment,the TRAP activity declined in a dose-de-pendent manner(P<0.01).The results of the biochemical tests showed that compared with the control group,the LPS+Erastin group had significantly elevated levels of ROS,Fe2+,and MDA,while the GSH level was significantly reduced(P<0.01),and compared with the LPS+Erastin group,the ROS,Fe2+,and MDA levels in the curcumin group decreased(P<0.01)and GSH levels increased(P<0.01).These effects were all dose-dependent.Transmission electron microscopy showed that compared with the LPS group,the LPS+Erastin group had reduced mitochondrial cristae and increased mem-brane density,while after treatment with curcumin,both these effects were reversed.The RT-qPCR and Western blot re-sults showed that compared with the control group,the mRNA and protein levels of p53 in the LPS+Erastin group were sig-nificantly increased,while those of of SLC7A11 and GPX4 were significantly reduced(P<0.01).After curcumin treat-ment,the p53 mRNA and protein levels were reduced while the levels of SLC7A11 and GPX4 were increased(P<0.01).CONCLUSION:Curcumin can inhibit lipopolysaccharide-induced differentiation of RAW264.7 cells into osteoclasts,and its mechanism may be related to the inhibition of the p53/SLC7A11/GPX4 signaling pathway.
李明;陈宗海;朱青;颜丽君;张宗星;邹权;陈龙菊
风湿性疾病发生与干预湖北省重点实验室,湖北民族大学医学部,湖北 恩施 445000川北医学院,四川 南充 637000
临床医学
骨质疏松铁死亡破骨细胞p53/SLC7A11/GPX4信号通路姜黄素
osteoporosisferroptosisosteoclastp53/SLC7A11/GPX4 signaling pathwaycurcumin
《中国病理生理杂志》 2024 (007)
1268-1275 / 8
国家自然科学基金资助项目(No.81260192);风湿性疾病发生与干预湖北省重点实验室第四批开放基金项目(No.OIR19007A);湖北民族大学研究生教育创新项目(No.MYK2024004)
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