中国病理生理杂志2024，Vol.40Issue(7)：1300-1306,7.DOI:10.3969/j.issn.1000-4718.2024.07.019
鹿茸多肽对顺铂所致卵巢早衰大鼠卵巢功能的保护作用及其机制
Protective effect of velvet antler polypeptide on ovarian function in cispla-tin-induced premature ovarian failure rats and its mechanism
摘要
Abstract
AIM:To investigate the effect and mechanism of velvet antler polypeptide(VAP)on premature ovarian failure induced by cisplatin(DDP)in rats.METHODS:Female rats with normal estrous cycle were randomly di-vided into the control,model,and VAP groups,with 10 rats in each group.Premature ovarian failure was induced in the model and VAP groups by intraperitoneal injection of DDP(4 mg·kg-1·d-1)for 20 consecutive days,and the VAP group was treated with VAP(400 mg·kg-1·d-1)for 20 consecutive days.Hematoxylin and eosin(HE)staining was used to ob-serve the morphological manifestations of the ovary,and the number of developing follicles at all levels was counted.En-zyme-linked immunosorbent assay(ELISA)was performed to detect serum estradiol(E2),follicle-stimulating hormone(FSH),and anti-Mullerian hormone(AMH)levels.The levels of glutathione(GSH)and malondialdehyde(MDA)in ovarian tissues were detected by biochemical methods.The level of reactive oxygen species(ROS)in ovarian tissue was detected by the probe labeling method.Prussian blue staining was performed to observe the iron accumulation in ovarian tissue,as well as the ferrous ion(Fe2+)level was detected by Fe2+content analysis kit.Immunohistochemistry and West-ern blot analysis were performed to detect the expressions of solute carrier family 7 member 11(SCL7A11),glutathione peroxidase 4(GPX4),and acyl-CoA synthetase long-chain family member 4(ACSL4)in the ovarian tissue.The fertility of rats was monitored in each group.RESULTS:Compared with control group,the model group showed ovarian atrophy,iron accumulation(P<0.01),reduced development of follicles at all levels and total follicles(P<0.05),increased atretic follicles(P<0.01),reduced litter size(P<0.01),decreased serum E2 and AMH levels(P<0.01),increased FSH levels(P<0.01),decreased GSH level and SCL7A11 and GPX4 protein expression levels in the ovarian tissue(P<0.01),and increased levels of Fe2+,MDA,and ROS and expression levels of ACSL4(P<0.01).Compared with model group,the VAP group had improvement in these aforementioned changes caused by DDP(P<0.01).CONCLUSION:Treatment with VAP can improve ovarian function,maintain normal follicle development,and improve fertility in cisplatin-induced premature ovarian failure rats,and the mechanism may be related to the inhibition of lipid peroxidation and ferroptosis.关键词
鹿茸多肽/顺铂/卵巢早衰/脂质过氧化/铁死亡Key words
velvet antler polypeptide/cisplatin/premature ovarian failure/lipid peroxidation/ferroptosis分类
医药卫生引用本文复制引用
史松,苏比努尔·买买提,尼比热·阿布都瓦依提,杨静,易金玲..鹿茸多肽对顺铂所致卵巢早衰大鼠卵巢功能的保护作用及其机制[J].中国病理生理杂志,2024,40(7):1300-1306,7.基金项目
新疆维吾尔自治区科技支疆项目(No.2020E02129) （No.2020E02129）
