中国草食动物科学2024，Vol.44Issue(4)：1-7,7.DOI:10.3969/j.issn.2095-3887.2024.00.071
基于RNA测序技术鉴定和分析绒山羊毛囊不同生长期mRNA的可变剪接
Identification and Analysis of Alternative Splicing of mRNA Related to Different Growth Stages of Cashmere Goat Hair Follicles Based on RNA Sequencing Technology
摘要
Abstract
Alternative splicing(AS)plays a crucial role in regulating gene expression post-transcriptionally.To explore key alternative splicing events linked to the cyclical growth of cashmere goat hair follicles,the skin transcriptome data of different growth stages were compared and analyzed.The results showed that 1 615,1 667,1 387 and 1 522 differen-tial alternative splicing were identified in transition periods Ⅰ,Ⅱ,Ⅲ and Ⅳ,respectively,corresponding to 1 202,1 268,1 073 and 1 170 genes.These genes were predominantly enriched in signaling pathways such as Notch,Hedge-hog,mTOR,AMPK,PI3K-Akt,Wnt,TGF-β,all known to be related to hair follicle development.Protein interaction network analysis coupled with enrichment analysis showed that alternative splicing produced by genes such as Ctnnb1,Gsk3b,Tgfbr1,Vegfa,Gli1 may contribute to the regulation of hair follicle cycle growth.In summary,alternative splic-ing is prevalent during the cyclical growth of cashmere goat hair follicles,with notable differences across developmen-tal stages.The key differentially alternative splicing genes and their related signaling pathways were identified,which provided valuable insights into the cycle growth and development of hair follicles in cashmere goats.关键词
绒山羊/毛囊周期性生长/可变剪接/转录组测序Key words
cashmere goat/hair follicles growth cycle/alternative splicing/RNA-seq分类
农业科技引用本文复制引用
李莹莹,董华娇,梁千千,张洁,李甜甜,许赫,马月辉,韩吉龙,杨敏..基于RNA测序技术鉴定和分析绒山羊毛囊不同生长期mRNA的可变剪接[J].中国草食动物科学,2024,44(4):1-7,7.基金项目
国家自然基金项目(32060745,31802045) （32060745,31802045）
石河子大学国际科技合作推进计划项目(GJHZ202307) （GJHZ202307）
