中国草食动物科学2024，Vol.44Issue(4)：45-54,10.DOI:10.3969/j.issn.2095-3887.2024.00.077
补饲对冷季放牧娟犏牛瘤胃菌群结构的影响
Effects of Cold Season Supplementation on Rumen Microbiota of Hybrid Offspring of Jersey Cattle and Yak
摘要
Abstract
In order to investigate the effect of cold season supplementation on the rumen flora of a hybrid breed of Jer-sey cattle and yak(Jersey cattle-yak),14 females in good condition at the age of 5 years were selected for supplementa-tion,divided into BQ group(before supplementation)and BH group(after supplementation),and the experimental pe-riod was 5 months.Fourteen rumen fluid samples were collected before and after the supplementation for 16S rRNA se-quencing.The results showed that a total of 3 581 307 raw reads were obtained from 28 rumen fluid samples,and 3 580 024 clean reads were obtained after quality control.Among them,there were 2 994 663 effective tags,and the ave-rage efficiency ranged from 83.27%to 83.95%.In addition,1 275 OTUs were co-existed in the rumen of Jersey cattle-yak in groups BQ and BH,and 259 and 317 individual OTUs were present in BQ and BH,respectively.The relative abundance of Proteobacteria,Desulfobacterota and Thermoplasmatota in the rumen of Jersey cattle-yak was signifi-cantly increased after supplementation(P＜0.05).At the genus level,the relative abundance of Acinetobacter,Comamo-nas and Lysinibacillus in group BH was significantly higher than those in group BQ(P＜0.05).PICRUST2 analysis re-vealed that 27 metabolism-related pathways differed significantly between groups BQ and BH(P＜0.05),with amino acid metabolism having the highest average abundance,followed by metabolism of cofactors,vitamins and carbohy-drate metabolism.In conclusion,supplemental feeding can increase the abundance of metabolism-related microflora and pathways in the rumen of Jersey cattle-yak,and thus improves its nutrient balance under cold-season grazing con-ditions.关键词
补饲/娟犏牛/瘤胃/菌群结构Key words
supplemental feeding/Jersey cattle-yak/rumen/bacterial flora structure分类
农业科技引用本文复制引用
车陇杰,魏立明,薛瑞林,石红梅,石斌刚,魏晓云..补饲对冷季放牧娟犏牛瘤胃菌群结构的影响[J].中国草食动物科学,2024,44(4):45-54,10.基金项目
甘肃省科技重大专项(21ZD10NA001) （21ZD10NA001）
甘肃省种业攻关项目(GZGG-2021-1) （GZGG-2021-1）
国家现代农业产业技术体系(CARS-37) （CARS-37）
