中国动脉硬化杂志2024，Vol.32Issue(7)：567-572,582,7.DOI:10.20039/j.cnki.1007-3949.2024.07.003
柚皮素通过调控平滑肌细胞TIMP-3表达促进动脉粥样硬化斑块稳定
Naringenin promotes atherosclerotic palque stability by regulating the expression of TIMP-3 in smooth muscle cells
摘要
Abstract
Aim To investigate the effects of naringenin on atherosclerotic plaque extracellular matrix remodeling and plaque stability.Methods Murine vascular smooth muscle cells were isolated and treated with various doges of naringenin.ApoE-/-mice were fed with high-fat diet and received naringenin by lavage for 16 weeks.Intraplaque nec-rotic core,contents of collagen and fibrous cap thickness were measured by Sirius red-Haematoxylin staining.Elastin was detected by Van Gieson staining.Matrix metalloproteinase(MMP)activity was determined by gelatin zymography and fluorescence-gelatin staining.Results Naringenin(50 μmol/L)increased signal tansducer and activator of transciption 6(STAT6)phosphorylation and promoted tissue inhibitor of metalloproteinase-3(TIMP-3)expression by 3.1-fold(P＜0.001).After naringenin(80 mg/kg)treatment,compared with the control group,the area of plaque necrotic core in aor-tic root decreased by 53％(P＜0.01),the thickness of fibrous caps increased by nearly 50％(P＜0.05),and the degree of elastic fiber degradation decreased.At the same time,naringenin promoted the expression of TIMP-3 in plaques,and corre-spondingly reduced the activity of MMP in plaques.Lentivirus mediated inhibition of TIMP-3 expression in vivo could reduce the protective effect of naringenin on plaque stability.Conclusion Naringin can increase the expression of TIMP-3 in smooth muscle cells,improve the composition of extracellular matrix,and promote the stability of atherosclerotic plaque.关键词
柚皮素/信号转导及转录活化因子6/组织型基质金属蛋白酶抑制剂3/动脉粥样硬化/斑块稳定性Key words
naringenin/signal tansducer and activator of transcipion 6/tissue inhibitor of metalloproteinase-3/atherosclerosis/plaque stability分类
医药卫生引用本文复制引用
何慧,董豆豆,丁萌,朱沅,姜天宇,许晓婷,欧海龙..柚皮素通过调控平滑肌细胞TIMP-3表达促进动脉粥样硬化斑块稳定[J].中国动脉硬化杂志,2024,32(7):567-572,582,7.基金项目
国家自然科学基金项目(32260232、32060219) （32260232、32060219）
贵州省科技基金项目(黔科合基础-ZK[2022]重点038) （黔科合基础-ZK[2022]重点038）
