局解手术学杂志2024，Vol.33Issue(7)：566-570,5.DOI:10.11659/jjssx.06E023048
血小板裂解液在大鼠脊髓损伤中的治疗作用
Therapeutic effect of platelet lysate on spinal cord injury in rats
摘要
Abstract
Objective To verify the therapeutic effect of platelet lysate(PL)on spinal cord injury in rats.Methods Thirty male SD rats were randomly divided into the sham operation group,the injury group and the treatment group,with 10 rats in each group.Rats in the sham operation group did not smash the spinal cord after exposure;rats in the injury group were established the rat spinal cord injury model by smashing the spinal cord with a 20 g of self-made smashing machine weights from a height of 5 cm for 5 seconds after exposure;rats in the treatment group were given intraperitoneal injection of 2 mL of PL once a day for 19 days after established the spinal cord injury model.The hind limb function was evaluated by Basso Beattie Bresnahan(BBB)score and slope experiment;six rats were randomly selected from each group,and the spinal cords of the rats were taken on the 7th day after surgery for HE staining and electron microscopy scanning to observe their pathological changes.Results There were statistically significant differences in BBB scores on the 3rd,7th and 14th days after surgery among the three groups(P<0.05),and the BBB scores of the treatment group were higher than those of the injury group(P<0.05).There were statistically significant differences in the results of slope experiment on the 7th and 14th days after surgery between the treatment group and the injury group(P<0.01).HE staining showed that the neuronal degeneration and necrosis decreased,the cell swelling improved and the microglial phagocytosis decreased in the treatment group compared with those in the injury group.The results of electron microscopy showed that the astrocyte cytoplasmic swelling and local cell membrane damage were alleviated,and the mitochondrial swelling and degeneration in the treatment group were relieved compared with those in the injury group.Conclusion PL can improve the hind limb function and reduce the pathological changes after spinal cord injury in rats,which has therapeutic effect on spinal cord injury in rats.关键词
脊髓损伤/血小板裂解液/脚印分析/斜坡实验Key words
spinal cord injury/platelet lysate/Basso Beattie Bresnahan/slope experiment分类
医药卫生引用本文复制引用
王玲,金红旭,刘颖,陈哲远,项良碧..血小板裂解液在大鼠脊髓损伤中的治疗作用[J].局解手术学杂志,2024,33(7):566-570,5.基金项目
辽宁省科技厅项目(2020JH2/10300157) （2020JH2/10300157）
