中国计量大学学报2024，Vol.35Issue(2)：341-348,8.DOI:10.3969/j.issn.2096-2835.2024.02.018
四烯菌素B标准样品的研制
Development of certified reference material of tetrin B
摘要
Abstract
Aims:This paper aims to develop a certified reference material(CRM)of tetrin B according to GB/T 150003-2008"Guidelines for the Work of Standard Samples(3)General Principles and Statistical Methods for Determining the Value of Standard Samples".Methods:Tetrin B was obtained from metabolites of Streptomyces diastatochramo genes 1628 by extraction,AB-8 and Sephardex LH-20 column chromatography.The structure of tetrin B was confirmed by UV,IR,MS and NMR analysis.Subsequently,tetrin B was divided into 100 bottles with 10 mg per bottle.The homogeneity and stability testing and cooperative certification were carried out by HPLC detection.Results:The homogeneity was good within 95% confidence interval by the F test statistical method.The sample was stable within a 12 months storage period at-20～-18 ℃.A cooperative certification was conducted with 8 qualified laboratories;and the certified purity value of tetrin B 99.37% with an expanded uncertainty of 0.22% .Conclusions:The reference material tetrin B conforms to the technical requirement of the certified reference material.The material can be used in the method validation and quality control regarding tetrin B.关键词
四烯菌素B/标准样品/均匀性/稳定性/不确定度Key words
tetrin B/standard sample/uniformity/stability/uncertainty分类
通用工业技术引用本文复制引用
雷承泽,刘一鹏,申屠旭萍,俞晓平..四烯菌素B标准样品的研制[J].中国计量大学学报,2024,35(2):341-348,8.基金项目
国家重点研发计划项目(No.2022YFF0607905),浙江省领雁计划项目(No.2022C02047),浙江省三农九方科技协作项目(No.2023SNJF034) （No.2022YFF0607905）
