中国临床药理学杂志2024，Vol.40Issue(13)：1898-1902,5.DOI:10.13699/j.cnki.1001-6821.2024.13.011
杜鹃素下调JAK2/STAT3通路改善高盐致脑基底动脉炎症和肌张力异常的研究
Farrerol improves the inflammation and abnormal muscle tone of cerebral basilar artery in mice induced by high salt via down-regulating JAK2/STAT3 pathway
摘要
Abstract
Objective To investigate the inhibitory effect of farrerol on inflammation and abnormal muscle tone of cerebral basilar artery in mice induced by high salt and its molecular mechanism based on the Janus kinase 2(JAK2)/Transcription activator 3(STAT3)pathway.Methods A total of fifty C57BL/6J mice were randomly divided into normal group(normal feeding),model group(high salt diet),experimental-L group(high salt diet+oral administration of 12.5 mg·kg-1·d-1 farrerol),experimental-M group(high salt diet+oral administration of 25 mg·kg-1·d-1 farrerol)and experimental-H group(high salt diet+oral administration of 50 mg·kg-1·d-1 farrerol).The model was prepared for 12 weeks.The contractile response of the cerebral basilar artery of mice in each group to vasoconstrictor was recorded with myographs.Enzyme linked immunosorbent assay(ELISA)were used to detect the levels of inflammatory factor.The protein expression levels of JAK2/STAT3 pathway related proteins were detected by Western blot.Results In the normal group,model group,experimental-L group,experimental-M group,experimental-H group,the contraction effects of the cerebral basilar artery to 60 mmol·L-1 potassium chloride(KCl)were(2.19±0.13),(2.66±0.11),(2.52±0.09),(2.41±0.08)and(2.25±0.10)mN;the contraction effects to 10-5 mol·L-1 vasopressiu(AVP)were(1.98±0.09),(2.46±0.08),(2.33±0.12),(2.11±0.10)and(2.05±0.06)mN;the contraction effects to 2.5 mmol·L-1 calcium chloride(CaCl2)were(1.77±0.08),(2.09±0.09),(2.03±0.08),(1.94±0.05)and(1.86±0.06)mN;in the serum,the levels of interleukin(IL)-1β were(10.10±3.21),(47.28±4.78),(40.16±3.98),(35.87±4.12)and(20.32±3.17)pg·mL-1;the levels of tumor necrosis factor-α(TNF-α)were(60.26±5.43),(134.32±4.15),(110.65±3.56),(90.79±5.25)and(81.54±6.23)pg·mL-1;the levels of chemokine ligand 3(CCL3)were(68.93±4.16),(146.37±5.73),(128.29±4.38),(100.25±6.82)and(84.16±3.89)pg·mL-1;the protein expression levels of JAK2 were 0.52±0.05,1.28±0.07,1.11±0.06,0.88±0.09 and 0.75±0.04;the protein expression levels of STAT3 were 0.58±0.07,1.93±0.10,1.62±0.04,1.34±0.06 and 0.88±0.09,respectively.The above indicators in the model group were significantly higher than the normal group(all P＜0.01);compared to the model group,the above indicators in the experimental-M and-H groups were significantly reduced(P＜0.05,P＜0.01).Conclusion Farrerol maybe improve the inflammation and abnormal muscle tone of cerebral basilar artery in mice induced by high salt by downregulating JAK2/STAT3 pathway.关键词
杜鹃素/Janus激酶2/转录激活子3/高盐/脑基底动脉/炎症/肌张力Key words
farrerol/Janus kinase 2/transcription activator 3/high salt/cerebral basilar artery/inflammation/muscle tone分类
医药卫生引用本文复制引用
侯晓敏,陈亮京,郝雨萱,张明升,秦小江..杜鹃素下调JAK2/STAT3通路改善高盐致脑基底动脉炎症和肌张力异常的研究[J].中国临床药理学杂志,2024,40(13):1898-1902,5.基金项目
国家自然科学基金青年基金资助项目(82204042) （82204042）
国家自然科学基金面上资助项目(82373622) （82373622）
国家卫生健康委员会尘肺病重点实验室开放课题资助项目(NHC202307) （NHC202307）
山西省自然科学基金面上资助项目(202103021224227) （202103021224227）
山西省科技创新人才团队专项基金资助项目(202304051001038) （202304051001038）
山西省科技合作交流专项基金资助项目(202204041101022) （202204041101022）
