中国临床药理学杂志2024，Vol.40Issue(13)：1908-1912,5.DOI:10.13699/j.cnki.1001-6821.2024.13.013
脉血康胶囊减轻IgA肾病大鼠肾纤维化的作用和机制
Effects and mechanism of Maixuekang capsule on reducing renal fibrosis in IgA nephropathy rats
摘要
Abstract
Objective To explore the effect of Maixuekang capsules on renal fibrosis and inhibition of Janus tyrosine protein kinase 2(JAK2)/signal transducer and activator of transcription family 3(STAT3)signal pathway in rats with immunoglobulin A nephropathy(IgAN).Methods The rat model of IgAN was established by modified 600 mg·kg-1 BSA+LPS 2 mL+CCL4 0.1 mL scheme,and the 24-hour urinary protein was detected.Fifty-five SD rats were randomly divided into 6 groups:Blank control group(distilled water+0.9％NaCl),model group(IgAN model),positive control group(10 mg·kg-1 telmisartan tablets after modeling),experimental-L,-M,-H groups(16,44,84 ATU·kg-1 Maixuekang capsules after modeling).The levels of serum creatinine(Scr),blood urea nitrogen(BUN),glutamic pyruvic transaminase(GPT)and glutamic oxalacetic transaminase(GOT)were determined by automatic biochemical analyzer.The morphology of kidney was observed by hematoxylin-eosin(HE)staining,and the deposition of IgA in rat kidney was detected by immunofluorescence.The expressions of α-SMA,TGF-β1,JAK2,STAT3 and P-STAT3 in renal tissue were detected by Western blot.Results The blood Scr of blank control group,model group,positive control and experimental-H groups were(41.42±2.30),(59.82±5.36),(50.28±3.18)and(50.47±7.19)μ mol·L-1,respectively;the 24-hour urinary protein were(2.35±1.21),(11.62±3.45),(5.01±4.36)and(8.14±4.19)mg·d-1,respectively;the relative expression of α-SMA protein were 0.48±0.10,1.00±0.00,0.71±0.13 and 0.57±0.22,respectively;the relative expression of TGF-β1 protein were 0.54±0.93,1.00±0.00,0.73±0.33 and 0.51±0.31,respectively;the relative expression of JAK2 protein were 0.36±0.22,1.00±0.00,0.82±0.36 and 0.57±0.28,respectively;p-STAT3/STAT3 were 0.40±0.19,1.00±0.00,0.46±0.18 and 0.31±0.18,respectively.Compared with the blank control group,the differences of above indexes in the model group were statistically significant(all P＜0.05).Compared with the model group,the differences of above indexes in the positive control and experimental-H groups were statistically significant(all P＜0.05).Conclusion Maixuekang capsules may reduce the level of renal fibrosis and protect renal function in rats with IgAN by inhibiting the activation of JAK2/STAT3 signal pathway.关键词
脉血康胶囊/免疫球蛋白A肾病/纤维化/Janus酪氨酸蛋白激酶2/信号转导与转录激活因子家族3Key words
Maixuekang capsules/immunoglobulin A nephropathy/fibrosis/Janus tyrosine protein kinase 2/signal transducer and activator of transcription family 3分类
医药卫生引用本文复制引用
陆瑾瑜,丁爱霞,陆晨..脉血康胶囊减轻IgA肾病大鼠肾纤维化的作用和机制[J].中国临床药理学杂志,2024,40(13):1908-1912,5.基金项目
科技创新领军人才基金资助项目(2022TSYCLJ0022) （2022TSYCLJ0022）
