中国临床药理学杂志2024，Vol.40Issue(13)：1928-1932,5.DOI:10.13699/j.cnki.1001-6821.2024.13.017
补肾活血方对复发性流产大鼠IL-17的影响
Effects of Bushen Huoxue formula on interleukin-17 in rats with recurrent spontaneous abortion
摘要
Abstract
Objective To observe the effect of Bushen Huoxue formula on interleukin-17(IL-17)in rats with recurrent spontaneous abortion(RSA).Methods RS A model rats were constructed by intraperitoneal injection of estradiol benzoate.Forty RSA model rats were randomly divided into model group,control group and experimental-L,-H groups,with 10 rats per group.Another 10 healthy pregnant rats were set as blank group.The experimental-L,-H groups were given 7.77 and 15.54 g·kg-1 of Bushen Huoxue formula solution by gavage.The control group was given 2.10 mg·kg-1of dydrogesterone solution by gavage.The blank group and the model group were given equal amount of 0.9％NaCl by gavage.The dose of administration for the five groups was 10 mL·kg-1,once a day,for 9 days.The number of live fetuses,embryo loss,and embryo loss rate in each group were observed.The proportion of Th 17 cells in the peripheral blood was detected by flow cytometry.The expression levels of interleukin-6(IL-6),IL-17 and IL-23 proteins in the decidual tissues were detected by Western blotting.Results The number of live fetuses in the experimental-H,control,model and blank groups were 11.50±2.84,11.50±3.10,6.30±1.25 and 12.50±3.24;the number of embryos lost were 1.80±0.42,1.90±0.57,4.90±1.37 and 0;the rates of embryo loss were(14.01±4.52)％,(14.79±6.06)％,(43.50±9.49)％and 0;the proportions of Th17 cells in the peripheral blood were(3.12±0.47)％,(3.10±0.59)％,(5.31±1.16)％and(2.54±0.71)％;the relative expression levels of IL-6 protein were 0.19±0.04,0.18±0.05,0.85±0.16 and 0.11±0.03;the relative expression levels of IL-17 protein were 0.28±0.04,0.29±0.05,0.84±0.12 and 0.09±0.01;the relative expression levels of IL-23 protein were 0.35±0.04,0.34±0.06,0.90±0.11 and 0.08±0.01,respectively.Comparing experimental-H group and model group,comparing control group and blank group,the above indexes were statistically significant(all P＜0.05).Conclusion Bushen Huoxue formula can reduce embryo loss,improve placental tissue pathology,reduce Th 17 cell proportion and its related cytokines IL-6,IL-17,IL-23 expression in rats with RSA.关键词
补肾活血方/复发性流产/白细胞介素-17/胚胎丢失Key words
Bushen Huoxue formula/recurrent spontaneous abortion/interleukin-17/embryo loss分类
医药卫生引用本文复制引用
彭瑶,陈瑾,梁艳,杨琦,沈丽梅,夏晓健..补肾活血方对复发性流产大鼠IL-17的影响[J].中国临床药理学杂志,2024,40(13):1928-1932,5.基金项目
国家中医药管理局第七批全国名老中医药专家学术经验继承工作资助项目(国中医药人教函[2022]76号) （国中医药人教函[2022]76号）
廖彩森全国名老中医药专家传承工作室(国中医药人教函[2022]75号) （国中医药人教函[2022]75号）
江西省卫生健康委科技计划基金资助项目(SKJP220218590) （SKJP220218590）
中国妇幼保健协会-华润三九中医中西医结合妇幼健康专项研究基金资助项目(ZXY2020B06) （ZXY2020B06）
