中国临床药理学杂志2024，Vol.40Issue(14)：2048-2052,5.DOI:10.13699/j.cnki.1001-6821.2024.14.010
苦参碱对神经母细胞瘤细胞增殖、迁移和侵袭的影响
Effects of matrine on the proliferation,migration,and invasion of neuroblastoma cells
摘要
Abstract
Objective To observe the effects of matrine on the proliferation,migration,and invasion of human neuroblastoma cells,and to investigate its potential mechanism.Methods This study was divided into AS experimental group(SK-N-AS cells treated with IC50 concentration of matrine),AS blank group(SK-N-AS cells cultured under normal conditions),AS control group(SK-N-AS cells treated with an equal amount of dimethyl sulfoxide),DZ experimental group(SK-N-DZ cells treated with IC50 concentration of matrine),DZ blank group(SK-N-DZ cells cultured under normal conditions),and DZ control group(SK-N-DZ cells treated with an equal amount of dimethyl sulfoxide).Scratch assay and Transwell chamber were used to measure the effect of matrine on the migration and invasion.The expression of E-cadherin,N-cadherin and Vimentin were tested by Western blot.Results After different intervention,the migration percentages of AS blank group,AS control group,AS experimental group,DZ blank group,DZ control group and DZ experimental group were(66.32±3.12)％,(65.27±3.44)％,(23.73±0.79)％,(46.25±4.68)％,(44.15±5.60)％and(16.77±3.52)％,respectively;the number of invasive cells were 870.45±19.32,865.32±23.39,492.74±16.81,1 198.10±43.71,1 203.03±71.91 and 891.69±42.62,respectively;the expression levels of E-cadherin protein were(100.00±11.72)％,(105.65±13.11)％,(477.20±29.71)％,(100.00±12.54)％,(97.78±12.77)％and(240.53±12.23)％,respectively;the expression levels of N-cadherin protein were(100.00±15.44)％,(103.90±10.76)％,(43.52±9.96)％,(100.00±10.12)％,(104.95±10.49)％and(38.39±8.70)％,respectively;Vimentin protein expression levels were(100.00±9.51)％,(97.39±11.33)％,(59.13±10.25)％,(100.00±13.20)％,(96.27±11.01)％and(47.67±9.48)％,respectively.There were statistically significant differences in the above indexes between the AS group and the AS blank group(P＜0.01,P＜0.001),and there were statistically significant differences between the above indexes in the DZ group and the DZ blank group(P＜0.01,P＜0.001).Conclusion Matrine inhibits the proliferation,migration,and invasion of neuroblastoma SK-N-AS and SK-N-DZ cells,potentially through suppressing epithelial-mesenchymal transition.关键词
苦参碱/神经母细胞瘤/迁移/侵袭/上皮间质转化Key words
matrine/neuroblastoma/migration/invasion/epithelial-mesenchymal transition分类
医药卫生引用本文复制引用
刘南京,王冬娟,刘方杰,黄文霞,邹琳,何晓燕..苦参碱对神经母细胞瘤细胞增殖、迁移和侵袭的影响[J].中国临床药理学杂志,2024,40(14):2048-2052,5.基金项目
重庆市科卫联合基金资助项目(2019ZY023126) （2019ZY023126）
重庆市中青年高端人才基金资助项目(202129) （202129）
重庆市研究生科研创新基金资助项目(CYB19154) （CYB19154）
