中国临床药理学杂志2024，Vol.40Issue(14)：2098-2102,5.DOI:10.13699/j.cnki.1001-6821.2024.14.020
毛蕊花糖苷对大强度运动大鼠骨骼肌耗氧量、线粒体酶活性的影响
Effects of verbascoside on oxygen consumption and mitochondrial enzyme activities in skeletal muscle of rats during intense exercise
摘要
Abstract
Objective To investigate the effects of verbascoside on oxygen consumption and mitochondrial enzyme activities in skeletal muscle of rats with intense exercise.Methods Fifty SD rats were randomly divided into control group,model group,experimental-L,-M,-H groups.Exercise training was performed in all groups except the control group.The control group and model group were given 2 mL of 0.9％NaCl,the experimental-L,-M,-H groups was given 20,40,80 mg·kg-1·d-1 verbascoside.The activities of Na+/K+-ATP and Ca2+/Mg2+-ATP were detected by kit.Protein expressions of optic atrophy 1(Opa1),mitochondrial fusion(Mfn)1 and Mfn2 were detected by Western blot.Results The Na+/K+-ATP of experimental-L,-M,-H groups,model group and blank group were(2.74±0.44),(3.50±0.38),(4.39±0.41),(2.13±0.32)and(5.75±0.42)U·mg-1;Ca2+/Mg2+-ATP were(4.01±0.32),(4.82±0.79),(6.57±0.70),(3.51±0.35)and(8.92±1.14)U·mg-1;Opa-1 were 0.40±0.05,0.52±0.04,0.69±0.09,0.25±0.06 and 0.78±0.11;Mfn1 were 0.47±0.06,0.59±0.07,0.74±0.08,0.32±0.05 and 0.89±0.12;Mfn2 were 0.51±0.07,0.65±0.06,0.83±0.06,0.35±0.06 and 1.02±0.13,respectively.There was statistical significance between control group and model group(all P＜0.05);there were statistically significant differences in the above indexes between the experimental-L,-M,-H groups and the model group(all P＜0.05).Conclusion Verminoside can improve the oxygen consumption and mitochondrial enzyme activity of skeletal muscle and increase the antioxidant capacity of rats with intense exercise.关键词
毛蕊花糖苷/大强度运动/骨骼肌耗氧量/线粒体酶活性/氧化应激Key words
verbascoside/high intensity exercise/oxygen consumption of skeletal muscle/mitochondrial enzyme activity/oxidative stress分类
医药卫生引用本文复制引用
苏泽海,秦玲,郝泉水..毛蕊花糖苷对大强度运动大鼠骨骼肌耗氧量、线粒体酶活性的影响[J].中国临床药理学杂志,2024,40(14):2098-2102,5.基金项目
湖北省卫生健康委科研项目立项基金资助项目(WJ2021M084) （WJ2021M084）
